Datasets

Title	Ribosomal RNA 2’-O-methylation dynamics during development and differentiation impact cell fate decisions.
Organism
Number of Samples	6
Release Date	2022/03/25 00:00
Sequencing Types
Protocol Details

Title
Authors	Häfner SJ,Jansson MD,Altinel K,Andersen KL,Abay-Nørgaard Z,Ménard P,Fontenas M,Sørensen DM,Gay DM,Arendrup FS,Tehler D,Krogh N,Nielsen H,Kraushar ML,Kirkeby A,Lund AH
Journal	Developmental cell
Publication Date	2023 Sep 11
Abstract	Translational regulation impacts both pluripotency maintenance and cell differentiation. To what degree the ribosome exerts control over this process remains unanswered. Accumulating evidence has demonstrated heterogeneity in ribosome composition in various organisms. 2'-O-methylation (2'-O-me) of rRNA represents an important source of heterogeneity, where site-specific alteration of methylation levels can modulate translation. Here, we examine changes in rRNA 2'-O-me during mouse brain development and tri-lineage differentiation of human embryonic stem cells (hESCs). We find distinct alterations between brain regions, as well as clear dynamics during cortex development and germ layer differentiation. We identify a methylation site impacting neuronal differentiation. Modulation of its methylation levels affects ribosome association of the fragile X mental retardation protein (FMRP) and is accompanied by an altered translation of WNT pathway-related mRNAs. Together, these data identify ribosome heterogeneity through rRNA 2'-O-me during early development and differentiation and suggest a direct role for ribosomes in regulating translation during cell fate acquisition. Copyright © 2023 Elsevier Inc. All rights reserved.
PMC
PMID	37473757
DOI

Häfner et al. 2023 (PRJNA819859)