Häfner et al. 2023 (PRJNA819859)
General Details
| Title | Ribosomal RNA 2’-O-methylation dynamics during development and differentiation impact cell fate decisions. |
|---|---|
| Organism | |
| Number of Samples | 6 |
| Release Date | 2022/03/25 00:00 |
| Sequencing Types | |
| Protocol Details |
Study Links
| GWIPS-viz | Trips-Viz |
|---|---|
Repository Details
| SRA | SRP365866 |
|---|---|
| ENA | SRP365866 |
| GEO | GSE199387 |
| BioProject | PRJNA819859 |
Publication
| Title | |
|---|---|
| Authors | Häfner SJ, Jansson MD, Altinel K, Andersen KL, Abay-Nørgaard Z, Ménard P, Fontenas M, Sørensen DM, Gay DM, Arendrup FS, Tehler D, Krogh N, Nielsen H, Kraushar ML, Kirkeby A, Lund AH |
| Journal | Developmental cell |
| Publication Date | 2023 Sep 11 |
| Abstract | Translational regulation impacts both pluripotency maintenance and cell differentiation. To what degree the ribosome exerts control over this process remains unanswered. Accumulating evidence has demonstrated heterogeneity in ribosome composition in various organisms. 2'-O-methylation (2'-O-me) of rRNA represents an important source of heterogeneity, where site-specific alteration of methylation levels can modulate translation. Here, we examine changes in rRNA 2'-O-me during mouse brain development and tri-lineage differentiation of human embryonic stem cells (hESCs). We find distinct alterations between brain regions, as well as clear dynamics during cortex development and germ layer differentiation. We identify a methylation site impacting neuronal differentiation. Modulation of its methylation levels affects ribosome association of the fragile X mental retardation protein (FMRP) and is accompanied by an altered translation of WNT pathway-related mRNAs. Together, these data identify ribosome heterogeneity through rRNA 2'-O-me during early development and differentiation and suggest a direct role for ribosomes in regulating translation during cell fate acquisition. Copyright © 2023 Elsevier Inc. All rights reserved. |
| PMC | |
| PMID | 37473757 |
| DOI |
| Run Accession | Study Accession | Scientific Name | Cell Line | Library Type | Treatment | GWIPS-viz | Trips-Viz | Reads | BAM | BigWig (F) | BigWig (R) | ||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| SRR18476754 | PRJNA819859 | Homo sapiens | H9 | Ribo-Seq | 0.0 |  |
 |
 |
 |
|
|
||
| SRR18476753 | PRJNA819859 | Homo sapiens | H9 | Ribo-Seq | 0.0 | |
|
|
|
|
|
||
| SRR18476752 | PRJNA819859 | Homo sapiens | H9 | Ribo-Seq | 0.0 | |
|
|
|
|
|
||
| SRR18476751 | PRJNA819859 | Homo sapiens | H9 | Ribo-Seq | 0.0 | |
|
|
|
|
|
||
| SRR18476750 | PRJNA819859 | Homo sapiens | H9 | Ribo-Seq | 0.0 | |
|
|
|
|
|
||
| SRR18476749 | PRJNA819859 | Homo sapiens | H9 | Ribo-Seq | 0.0 | |
|
|
|
|
|
||
| Run Accession | Study Accession | Scientific Name | Cell Line | Library Type | Treatment | GWIPS-viz | Trips-Viz | Reads | BAM | BigWig (F) | BigWig (R) |
ⓘ For more Information on the columns shown here see: About