Zhao et al. 2022 (PRJNA838843)

General Details

Title Multifarious translational regulation during replicative aging in yeast
Organism
Number of Samples 5
Release Date 2022/05/17 00:00
Sequencing Types
Protocol Details

Study Links

Repository Details

SRA SRP375616
ENA SRP375616
GEO GSE203147
BioProject PRJNA838843

Publication

Title
Authors Zhao T,Chida A,Shichino Y,Choi D,Mizunuma M,Iwasaki S,Ohya Y
Journal Journal of fungi (Basel, Switzerland)
Publication Date 2022 Sep 5
Abstract Protein synthesis is strictly regulated during replicative aging in yeast, but global translational regulation during replicative aging is poorly characterized. To conduct ribosome profiling during replicative aging, we collected a large number of dividing aged cells using a miniature chemostat aging device. Translational efficiency, defined as the number of ribosome footprints normalized to transcript abundance, was compared between young and aged cells for each gene. We identified more than 700 genes with changes greater than twofold during replicative aging. Increased translational efficiency was observed in genes involved in DNA repair and chromosome organization. Decreased translational efficiency was observed in genes encoding ribosome components, transposon Ty1 and Ty2 genes, transcription factor HAC1 gene associated with the unfolded protein response, genes involved in cell wall synthesis and assembly, and ammonium permease genes. Our results provide a global view of translational regulation during replicative aging, in which the pathways involved in various cell functions are translationally regulated and cause diverse phenotypic changes.
PMC PMC9500732
PMID 36135663
DOI
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)
SRR19240767 PRJNA838843 Saccharomyces cerevisiae Yeast cells
SRR19240770 PRJNA838843 Saccharomyces cerevisiae Yeast cells
SRR19240766 PRJNA838843 Saccharomyces cerevisiae Yeast cells
SRR19240765 PRJNA838843 Saccharomyces cerevisiae Yeast cells
SRR19240764 PRJNA838843 Saccharomyces cerevisiae Yeast cells
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)

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