Gasparski et al. 2024 (PRJNA853863)
General Details
| Title | mRNA Location and Translation Rate Determine Protein Targeting to Dual Destinations |
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| Organism | |
| Number of Samples | 2 |
| Release Date | 2022/06/28 00:00 |
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| Protocol Details |
Study Links
| GWIPS-viz | Trips-Viz |
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Repository Details
| SRA | SRP384078 |
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| ENA | SRP384078 |
| GEO | 0.0 |
| BioProject | PRJNA853863 |
Publication
| Title | |
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| Authors | Gasparski AN, Moissoglu K, Pallikkuth S, Meydan S, Guydosh NR, Mili S |
| Journal | Molecular cell |
| Publication Date | 2023 Aug 3 |
| Abstract | Numerous proteins are targeted to two or multiple subcellular destinations where they exert distinct functional consequences. The balance between such differential targeting is thought to be determined post-translationally, relying on protein sorting mechanisms. Here, we show that mRNA location and translation rate can also determine protein targeting by modulating protein binding to specific interacting partners. Peripheral localization of the NET1 mRNA and fast translation lead to higher cytosolic retention of the NET1 protein by promoting its binding to the membrane-associated scaffold protein CASK. By contrast, perinuclear mRNA location and/or slower translation rate favor nuclear targeting by promoting binding to importins. This mRNA location-dependent mechanism is modulated by physiological stimuli and profoundly impacts NET1 function in cell motility. These results reveal that the location of protein synthesis and the rate of translation elongation act in coordination as a 'partner-selection' mechanism that robustly influences protein distribution and function. Published by Elsevier Inc. |
| PMC | PMC10530421 |
| PMID | 37506697 |
| DOI |
| Run Accession | Study Accession | Scientific Name | Cell Line | Library Type | Treatment | GWIPS-viz | Trips-Viz | Reads | BAM | BigWig (F) | BigWig (R) | ||
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| SRR19892207 | PRJNA853863 | Homo sapiens | HEK293 | Ribo-Seq | 0.0 |  |
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| SRR19892206 | PRJNA853863 | Homo sapiens | HEK293 | Ribo-Seq | 0.0 | |
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| Run Accession | Study Accession | Scientific Name | Cell Line | Library Type | Treatment | GWIPS-viz | Trips-Viz | Reads | BAM | BigWig (F) | BigWig (R) |
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