Datasets

Title	mRNA expression and translation regulated by the prion-like domain of Ilf3 in the mouse amygdala under chronic stress
Organism
Number of Samples	8
Release Date	2022/07/27 00:00
Sequencing Types
Protocol Details

Title
Authors	Yamashita A,Shichino Y,Fujii K,Koshidaka Y,Adachi M,Sasagawa E,Mito M,Nakagawa S,Iwasaki S,Takao K,Shiina N
Journal	iScience
Publication Date	2023 Mar 17
Abstract	The prion-like domain (PrLD) is a class of intrinsically disordered regions. Although its propensity to form condensates has been studied in the context of neurodegenerative diseases, the physiological role of PrLD remains unclear. Here, we investigated the role of PrLD in the RNA-binding protein NFAR2, generated by a splicing variant of the Ilf3 gene. Removal of the PrLD in mice did not impair the function of NFAR2 required for survival, but did affect the responses to chronic water immersion and restraint stress (WIRS). The PrLD was required for WIRS-sensitive nuclear localization of NFAR2 and WIRS-induced changes in mRNA expression and translation in the amygdala, a fear-related brain region. Consistently, the PrLD conferred resistance to WIRS in fear-associated memory formation. Our study provides insights into the PrLD-dependent role of NFAR2 for chronic stress adaptation in the brain. © 2023 The Author(s).
PMC	PMC9982275
PMID	36876121
DOI

Yamashita et al. 2023 (PRJNA862900)