Breznak et al. 2022 (PRJNA865860)
General Details
| Title | Ribosome profiling in Drosophila ovary enriched for late-stage oocytes (late-ovary) |
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| Organism | |
| Number of Samples | 3 |
| Release Date | 2022/08/03 00:00 |
| Sequencing Types | |
| Protocol Details |
Study Links
| GWIPS-viz | Trips-Viz |
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Repository Details
| SRA | SRP389835 |
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| ENA | SRP389835 |
| GEO | |
| BioProject | PRJNA865860 |
Publication
| Title | |
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| Authors | Breznak SM,Peng Y,Deng L,Kotb NM,Flamholz Z,Rapisarda IT,Martin ET,LaBarge KA,Fabris D,Gavis ER,Rangan P |
| Journal | Science advances |
| Publication Date | 2023 Jun 23 |
| Abstract | Stem cells in many systems, including Drosophila germline stem cells (GSCs), increase ribosome biogenesis and translation during terminal differentiation. Here, we show that the H/ACA small nuclear ribonucleoprotein (snRNP) complex that promotes pseudouridylation of ribosomal RNA (rRNA) and ribosome biogenesis is required for oocyte specification. Reducing ribosome levels during differentiation decreased the translation of a subset of messenger RNAs that are enriched for CAG trinucleotide repeats and encode polyglutamine-containing proteins, including differentiation factors such as RNA-binding Fox protein 1. Moreover, ribosomes were enriched at CAG repeats within transcripts during oogenesis. Increasing target of rapamycin (TOR) activity to elevate ribosome levels in H/ACA snRNP complex-depleted germlines suppressed the GSC differentiation defects, whereas germlines treated with the TOR inhibitor rapamycin had reduced levels of polyglutamine-containing proteins. Thus, ribosome biogenesis and ribosome levels can control stem cell differentiation via selective translation of CAG repeat-containing transcripts. |
| PMC | PMC10284551 |
| PMID | 37343092 |
| DOI |
| Run Accession | Study Accession | Scientific Name | Cell Line | Library Type | Treatment | GWIPS-viz | Trips-Viz | Reads | BAM | BigWig (F) | BigWig (R) | ||
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| SRR20820095 | PRJNA865860 | Drosophila melanogaster | Late-stage oocytes | Ribo-Seq | Frozen |  |
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| SRR20820094 | PRJNA865860 | Drosophila melanogaster | Late-stage oocytes | Ribo-Seq | Frozen | |
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| SRR20820093 | PRJNA865860 | Drosophila melanogaster | Late-stage oocytes | Ribo-Seq | Frozen | |
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| Run Accession | Study Accession | Scientific Name | Cell Line | Library Type | Treatment | GWIPS-viz | Trips-Viz | Reads | BAM | BigWig (F) | BigWig (R) |
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