Bohlen et al. 2023 (PRJNA870413)

General Details

Title PRRC2 proteins are translation factors that promote leaky scanning
Organism
Number of Samples 6
Release Date 2022/08/17 00:00
Sequencing Types
Protocol Details

Study Links

Repository Details

SRA SRP392653
ENA SRP392653
GEO GSE211440
BioProject PRJNA870413

Publication

Title
Authors Bohlen J,Roiuk M,Neff M,Teleman AA
Journal Nucleic acids research
Publication Date 2023 Apr 24
Abstract Roughly half of animal mRNAs contain upstream open reading frames (uORFs). These uORFs can represent an impediment to translation of the main ORF since ribosomes usually bind the mRNA cap at the 5' end and then scan for ORFs in a 5'-to-3' fashion. One way for ribosomes to bypass uORFs is via leaky scanning, whereby the ribosome disregards the uORF start codon. Hence leaky scanning is an important instance of post-transcriptional regulation that affects gene expression. Few molecular factors regulating or facilitating this process are known. Here we show that the PRRC2 proteins PRRC2A, PRRC2B and PRRC2C impact translation initiation. We find that they bind eukaryotic translation initiation factors and preinitiation complexes, and are enriched on ribosomes translating mRNAs with uORFs. We find that PRRC2 proteins promote leaky scanning past translation start codons, thereby promoting translation of mRNAs containing uORFs. Since PRRC2 proteins have been associated with cancer, this provides a mechanistic starting point for understanding their physiological and pathophysiological roles. © The Author(s) 2023. Published by Oxford University Press on behalf of Nucleic Acids Research.
PMC PMC10123092
PMID 36869665
DOI
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)
SRR21102203 PRJNA870413 Homo sapiens LSU
SRR21102202 PRJNA870413 Homo sapiens LSU
SRR21102201 PRJNA870413 Homo sapiens LSU
SRR21102195 PRJNA870413 Homo sapiens LSU
SRR21102194 PRJNA870413 Homo sapiens LSU
SRR21102193 PRJNA870413 Homo sapiens LSU
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)

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