Datasets

Title	PRRC2 proteins are translation factors that promote leaky scanning
Organism
Number of Samples	6
Release Date	2022/08/17 00:00
Sequencing Types
Protocol Details

Title
Authors	Bohlen J, Roiuk M, Neff M, Teleman AA
Journal	Nucleic acids research
Publication Date	2023 Apr 24
Abstract	Roughly half of animal mRNAs contain upstream open reading frames (uORFs). These uORFs can represent an impediment to translation of the main ORF since ribosomes usually bind the mRNA cap at the 5' end and then scan for ORFs in a 5'-to-3' fashion. One way for ribosomes to bypass uORFs is via leaky scanning, whereby the ribosome disregards the uORF start codon. Hence leaky scanning is an important instance of post-transcriptional regulation that affects gene expression. Few molecular factors regulating or facilitating this process are known. Here we show that the PRRC2 proteins PRRC2A, PRRC2B and PRRC2C impact translation initiation. We find that they bind eukaryotic translation initiation factors and preinitiation complexes, and are enriched on ribosomes translating mRNAs with uORFs. We find that PRRC2 proteins promote leaky scanning past translation start codons, thereby promoting translation of mRNAs containing uORFs. Since PRRC2 proteins have been associated with cancer, this provides a mechanistic starting point for understanding their physiological and pathophysiological roles. © The Author(s) 2023. Published by Oxford University Press on behalf of Nucleic Acids Research.
PMC	PMC10123092
PMID	36869665
DOI

Bohlen et al. 2023 (PRJNA870413)