Rao et al. 2022 (PRJNA872137)
General Details
| Title | The Translation Initiation Factor Homolog, eif4e1c, Regulates Cardiomyocyte Metabolism and Proliferation During Heart Regeneration. |
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| Organism | |
| Number of Samples | 4 |
| Release Date | 2022/08/22 00:00 |
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| Protocol Details |
Study Links
| GWIPS-viz | Trips-Viz |
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Repository Details
| SRA | SRP394145 |
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| ENA | SRP394145 |
| GEO | |
| BioProject | PRJNA872137 |
Publication
| Title | |
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| Authors | Rao A,Lyu B,Jahan I,Lubertozzi A,Zhou G,Tedeschi F,Jankowsky E,Kang J,Carstens B,Poss KD,Baskin K,Goldman JA |
| Journal | Development (Cambridge, England) |
| Publication Date | 2023 Oct 15 |
| Abstract | The eIF4E family of translation initiation factors bind 5' methylated caps and act as the limiting step for mRNA translation. The canonical eIF4E1A is required for cell viability, yet other related eIF4E families exist and are utilized in specific contexts or tissues. Here, we describe a family called Eif4e1c, for which we find roles during heart development and regeneration in zebrafish. The Eif4e1c family is present in all aquatic vertebrates but is lost in all terrestrial species. A core group of amino acids shared over 500 million years of evolution forms an interface along the protein surface, suggesting that Eif4e1c functions in a novel pathway. Deletion of eif4e1c in zebrafish caused growth deficits and impaired survival in juveniles. Mutants surviving to adulthood had fewer cardiomyocytes and reduced proliferative responses to cardiac injury. Ribosome profiling of mutant hearts demonstrated changes in translation efficiency of mRNA for genes known to regulate cardiomyocyte proliferation. Although eif4e1c is broadly expressed, its disruption had most notable impact on the heart and at juvenile stages. Our findings reveal context-dependent requirements for translation initiation regulators during heart regeneration. © 2023. Published by The Company of Biologists Ltd. |
| PMC | PMC10281269 |
| PMID | 37306388 |
| DOI |
| Run Accession | Study Accession | Scientific Name | Cell Line | Library Type | Treatment | GWIPS-viz | Trips-Viz | Reads | BAM | BigWig (F) | BigWig (R) | ||
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| SRR21218378 | PRJNA872137 | Danio rerio | Wt | Ribo-Seq |  |
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| SRR21218377 | PRJNA872137 | Danio rerio | Wt | Ribo-Seq | |
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| SRR21218374 | PRJNA872137 | Danio rerio | Mutant | Ribo-Seq | |
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| SRR21218373 | PRJNA872137 | Danio rerio | Mutant | Ribo-Seq | |
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| Run Accession | Study Accession | Scientific Name | Cell Line | Library Type | Treatment | GWIPS-viz | Trips-Viz | Reads | BAM | BigWig (F) | BigWig (R) |
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