Datasets

Title	Differential ribosome engagement profiling between undifferentiated and neuron-differentiated P19 cells
Organism
Number of Samples	8
Release Date	2022/10/04 00:00
Sequencing Types
Protocol Details

Title
Authors	Baptissart M, Papas BN, Chi RA, Li Y, Lee D, Puviindran B, Morgan M
Journal	iScience
Publication Date	2023 Sep 15
Abstract	Cell differentiation is associated with global changes in translational activity. Here, we characterize how mRNA poly(A) tail processing supports this dynamic. We observe that decreased translation during neuronal differentiation of P19 cells correlates with the downregulation of 5'-terminal oligopyrimidine (TOP) transcripts which encode the translational machinery. Despite their downregulation, TOP transcripts remain highly stable and show increased translation as cells differentiate. Changes in TOP mRNA metabolism are reflected by their accumulation with poly(A) tails ∼60-nucleotide (nt) long. The dynamic changes in poly(A) processing can be partially recapitulated by depleting LARP1 or activating the mTOR pathway in undifferentiated cells. Although mTOR-induced accumulation of TOP mRNAs with tails ∼60-nt long does not trigger differentiation, it is associated with reduced proliferation of neuronal progenitors. We propose that while TOP mRNAs are transcriptionally silenced, their post-transcriptional regulation mediated by a specific poly(A) processing ensures an adequate supply of ribosomes to complete differentiation.
PMC	PMC10448114
PMID	37636056
DOI

Baptissart et al. 2023 (PRJNA887109)