Zhang et al. 2024 (PRJNA915110)

Title	Spermidine-eIF5A axis is essential for muscle stem cell activation via translational control (Ribo-Seq)
Organism
Number of Samples	4
Release Date	2022/12/22 00:00
Sequencing Types
Protocol Details

GWIPS-viz	Trips-Viz

Title
Authors	Zhang Q,Han W,Wu R,Deng S,Meng J,Yang Y,Li L,Sun M,Ai H,Chen Y,Liu Q,Gao T,Niu X,Liu H,Zhang L,Zhang D,Chen M,Yin P,Zhang L,Tang P,Zhu D,Zhang Y,Li H
Journal	Cell discovery
Publication Date	2024 Sep 10
Abstract	Adult skeletal muscle stem cells, also known satellite cells (SCs), are quiescent and activate in response to injury. However, the activation mechanisms of quiescent SCs (QSCs) remain largely unknown. Here, we investigated the metabolic regulation of SC activation by identifying regulatory metabolites that promote SC activation. Using targeted metabolomics, we found that spermidine acts as a regulatory metabolite to promote SC activation and muscle regeneration in mice. Mechanistically, spermidine activates SCs via generating hypusinated eIF5A. Using SC-specific eIF5A-knockout (KO) and Myod-KO mice, we further found that eIF5A is required for spermidine-mediated SC activation by controlling MyoD translation. More significantly, depletion of eIF5A in SCs results in impaired muscle regeneration in mice. Together, the findings of our study define a novel mechanism that is essential for SC activation and acts via spermidine-eIF5A-mediated MyoD translation. Our findings suggest that the spermidine-eIF5A axis represents a promising pharmacological target in efforts to activate endogenous SCs for the treatment of muscular disease. © 2024. The Author(s).
PMC	PMC11383958
PMID	39251577
DOI

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