Jariyasakulroj et al. 2023 (PRJNA926978)
General Details
| Title | Ribosome biogenesis controls suture mesenchymal stem cell fate via selective regulation ofcomplement pathway in to prevent craniosynostosis [Ribo-seq] |
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| Organism | |
| Number of Samples | 4 |
| Release Date | 2023/01/24 00:00 |
| Sequencing Types | |
| Protocol Details |
Study Links
| GWIPS-viz | Trips-Viz |
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Repository Details
| SRA | SRP418884 |
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| ENA | SRP418884 |
| GEO | |
| BioProject | PRJNA926978 |
Publication
| Title | |
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| Authors | Jariyasakulroj S,Zhang W,Bai J,Zhang M,Lu Z,Chen JF |
| Journal | Stem cell reports |
| Publication Date | 2023 Dec 12 |
| Abstract | Disruption of global ribosome biogenesis selectively affects craniofacial tissues with unclear mechanisms. Craniosynostosis is a congenital craniofacial disorder characterized by premature fusion of cranial suture(s) with loss of suture mesenchymal stem cells (MSCs). Here we focused on ribosomopathy disease gene Snord118, which encodes a small nucleolar RNA (snoRNA), to genetically disturb ribosome biogenesis in suture MSCs using mouse and human induced pluripotent stem cell (iPSC) models. Snord118 depletion exhibited p53 activation, increased cell death, reduced proliferation, and premature osteogenic differentiation of MSCs, leading to suture growth and craniosynostosis defects. Mechanistically, Snord118 deficiency causes translational dysregulation of ribosomal proteins and downregulation of complement pathway genes. Further complement pathway disruption by knockout of complement C3a receptor 1 (C3ar1) exacerbated MSC and suture defects in mutant mice, whereas activating the complement pathway rescued MSC cell fate and suture growth defects. Thus, ribosome biogenesis controls MSC fate via the complement pathway to prevent craniosynostosis. Copyright © 2023 The Author(s). Published by Elsevier Inc. All rights reserved. |
| PMC | PMC10724072 |
| PMID | 37977145 |
| DOI |
| Run Accession | Study Accession | Scientific Name | Cell Line | Library Type | Treatment | GWIPS-viz | Trips-Viz | Reads | BAM | BigWig (F) | BigWig (R) | ||
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| SRR23204134 | PRJNA926978 | Homo sapiens | human iPS derived mesenchymal stem cellsmesenchymal stem cells | Ribo-Seq |  |
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| SRR23204133 | PRJNA926978 | Homo sapiens | human iPS derived mesenchymal stem cellsmesenchymal stem cells | Ribo-Seq | |
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| SRR23204132 | PRJNA926978 | Homo sapiens | human iPS derived mesenchymal stem cellsmesenchymal stem cells | Ribo-Seq | |
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| SRR23204131 | PRJNA926978 | Homo sapiens | human iPS derived mesenchymal stem cellsmesenchymal stem cells | Ribo-Seq | |
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| Run Accession | Study Accession | Scientific Name | Cell Line | Library Type | Treatment | GWIPS-viz | Trips-Viz | Reads | BAM | BigWig (F) | BigWig (R) |
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