Yang et al. 2023 (PRJNA956972)

General Details

Title Germ-cell specific eIF4E1b regulates maternal mRNA translation to ensure zygotic genome activation [Ribo-seq]
Organism
Number of Samples 15
Release Date 2023/04/18 00:00
Sequencing Types
Protocol Details

Study Links

Repository Details

SRA SRP433275
ENA SRP433275
GEO GSE230019
BioProject PRJNA956972

Publication

Title
Authors Yang G,Xin Q,Feng I,Wu D,Dean J
Journal Genes & development
Publication Date 2023 May 1
Abstract Translation of maternal mRNAs is detected before transcription of zygotic genes and is essential for mammalian embryo development. How certain maternal mRNAs are selected for translation instead of degradation and how this burst of translation affects zygotic genome activation remain unknown. Using gene-edited mice, we document that the oocyte-specific eukaryotic translation initiation factor 4E family member 1b (eIF4E1b) is the regulator of maternal mRNA expression that ensures subsequent reprogramming of the zygotic genome. In oocytes, eIF4E1b binds to transcripts encoding translation machinery proteins, chromatin remodelers, and reprogramming factors to promote their translation in zygotes and protect them from degradation. The protein products are thought to establish an open chromatin landscape in one-cell zygotes to enable transcription of genes required for cleavage stage development. Our results define a program for rapid resetting of the zygotic epigenome that is regulated by maternal mRNA expression and provide new insights into the mammalian maternal-to-zygotic transition. Published by Cold Spring Harbor Laboratory Press.
PMC PMC10270193
PMID 37257918
DOI
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)
SRR24207638 PRJNA956972 Mus musculus Ribo-Seq Frozen
SRR24207637 PRJNA956972 Mus musculus Ribo-Seq Frozen
SRR24207634 PRJNA956972 Mus musculus Ribo-Seq Frozen
SRR24207633 PRJNA956972 Mus musculus Ribo-Seq Frozen
SRR24207635 PRJNA956972 Mus musculus Ribo-Seq Frozen
SRR24207632 PRJNA956972 Mus musculus Ribo-Seq Frozen
SRR24207636 PRJNA956972 Mus musculus Ribo-Seq Frozen
SRR24207631 PRJNA956972 Mus musculus Ribo-Seq Frozen
SRR24207630 PRJNA956972 Mus musculus Ribo-Seq Frozen
SRR24207627 PRJNA956972 Mus musculus Ribo-Seq Frozen
SRR24207626 PRJNA956972 Mus musculus Ribo-Seq Frozen
SRR24207628 PRJNA956972 Mus musculus Ribo-Seq Frozen
SRR24207625 PRJNA956972 Mus musculus Ribo-Seq Frozen
SRR24207629 PRJNA956972 Mus musculus Ribo-Seq Frozen
SRR24207624 PRJNA956972 Mus musculus Ribo-Seq Frozen
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)

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