Shi et al. 2024 (PRJNA990710)

General Details

Title The tRNA Gm18 Methyltransferase TARBP1 Promotes Hepatocellular Carcinoma Progression via Metabolic Reprogramming of Glutamine [Ribo-seq]
Organism
Number of Samples 4
Release Date 2023/07/03 00:00
Sequencing Types
Protocol Details

Study Links

Repository Details

SRA SRP447126
ENA SRP447126
GEO 0.0
BioProject PRJNA990710

Publication

Title
Authors Shi X, Zhang Y, Wang Y, Wang J, Gao Y, Wang R, Wang L, Xiong M, Cao Y, Ou N, Liu Q, Ma H, Cai J, Chen H
Journal Cell death and differentiation
Publication Date 2024 Sep
Abstract Cancer cells rely on metabolic reprogramming to sustain the prodigious energetic requirements for rapid growth and proliferation. Glutamine metabolism is frequently dysregulated in cancers and is being exploited as a potential therapeutic target. Using CRISPR/Cas9 interference (CRISPRi) screening, we identified TARBP1 (TAR (HIV-1) RNA Binding Protein 1) as a critical regulator involved in glutamine reliance of cancer cell. Consistent with this discovery, TARBP1 amplification and overexpression are frequently observed in various cancers. Knockout of TARBP1 significantly suppresses cell proliferation, colony formation and xenograft tumor growth. Mechanistically, TARBP1 selectively methylates and stabilizes a small subset of tRNAs, which promotes efficient protein synthesis of glutamine transporter-ASCT2 (also known as SLC1A5) and glutamine import to fuel the growth of cancer cell. Moreover, we found that the gene expression of TARBP1 and ASCT2 are upregulated in combination in clinical cohorts and their upregulation is associated with unfavorable prognosis of HCC (hepatocellular carcinoma). Taken together, this study reveals the unexpected role of TARBP1 in coordinating the tRNA availability and glutamine uptake during HCC progression and provides a potential target for tumor therapy. © 2024. The Author(s), under exclusive licence to ADMC Associazione Differenziamento e Morte Cellulare.
PMC PMC11368932
PMID 38867004
DOI
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)
SRR25115689 PRJNA990710 Homo sapiens PLC/PRF/5NA_Cancer cell Ribo-Seq 0.0
SRR25115688 PRJNA990710 Homo sapiens PLC/PRF/5NA_Cancer cell Ribo-Seq 0.0
SRR25115687 PRJNA990710 Homo sapiens PLC/PRF/5NA_Cancer cell Ribo-Seq 0.0
SRR25115686 PRJNA990710 Homo sapiens PLC/PRF/5NA_Cancer cell Ribo-Seq 0.0
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)

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