Datasets

Title	Rapid and synchronous chemical induction of replicative-like senescence via a small molecule inhibitor [Ribo-seq]
Organism
Number of Samples	9
Release Date	2023/07/25 00:00
Sequencing Types
Protocol Details

Title
Authors	Palikyras S, Sofiadis K, Stavropoulou A, Danieli-Mackay A, Varamogianni-Mamatsi V, Hörl D, Nasiscionyte S, Zhu Y, Papadionysiou I, Papadakis A, Josipovic N, Zirkel A, O'Connell A, Loughran G, Keane J, Michel A, Wagner W, Beyer A, Harz H, Leonhardt H, Lukinavicius G, Nikolaou C, Papantonis A
Journal	Aging cell
Publication Date	2024 Apr
Abstract	Cellular senescence is acknowledged as a key contributor to organismal ageing and late-life disease. Though popular, the study of senescence in vitro can be complicated by the prolonged and asynchronous timing of cells committing to it and by its paracrine effects. To address these issues, we repurposed a small molecule inhibitor, inflachromene (ICM), to induce senescence to human primary cells. Within 6 days of treatment with ICM, senescence hallmarks, including the nuclear eviction of HMGB1 and -B2, are uniformly induced across IMR90 cell populations. By generating and comparing various high throughput datasets from ICM-induced and replicative senescence, we uncovered a high similarity of the two states. Notably though, ICM suppresses the pro-inflammatory secretome associated with senescence, thus alleviating most paracrine effects. In summary, ICM rapidly and synchronously induces a senescent-like phenotype thereby allowing the study of its core regulatory program without confounding heterogeneity. © 2024 The Authors. Aging Cell published by Anatomical Society and John Wiley & Sons Ltd.
PMC	PMC11019153
PMID	38196311
DOI

Palikyras et al. 2024 (PRJNA998457)