Datasets

Title	Endonucleolytic RNA cleavage drives changes in gene expression during the innate immune response [ribosome profiling]
Organism
Number of Samples	10
Release Date	2023/09/27 00:00
Sequencing Types
Protocol Details

Title
Authors	Karasik A,Lorenzi HA,DePass AV,Guydosh NR
Journal	Cell reports
Publication Date	2024 Jun 25
Abstract	Viral infection triggers several double-stranded RNA (dsRNA) sensors that lead to changes in gene expression in the cell. One of these sensors activates an endonuclease, ribonuclease L (RNase L), that cleaves single-stranded RNA. However, how the resultant widespread RNA fragmentation affects gene expression is not fully understood. Here, we show that this fragmentation induces the ribotoxic stress response via ZAKα, potentially through stalled ribosomes and/or ribosome collisions. The p38 and JNK pathways that are activated as part of this response promote outcomes that inhibit the virus, such as programmed cell death. We also show that RNase L limits the translation of stress-responsive genes. Intriguingly, we found that the activity of the generic endonuclease, RNase A, recapitulates many of the same molecular phenotypes as activated RNase L, demonstrating how widespread RNA cleavage can evoke an antiviral program. Published by Elsevier Inc.
PMC	PMC11251458
PMID	38823018
DOI

Karasik et al. 2024 (PRJNA1021597)