Yu et al. 2024 (PRJNA1103142)
General Details
| Title | Translatomics reveals the role of dietary calcium addition in regulating muscle fat deposition in pigs |
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| Organism | |
| Number of Samples | 6 |
| Release Date | 2024/04/22 00:00 |
| Sequencing Types | |
| Protocol Details |
Study Links
| GWIPS-viz | Trips-Viz |
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Repository Details
| SRA | SRP503331 |
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| ENA | SRP503331 |
| GEO | 0.0 |
| BioProject | PRJNA1103142 |
Publication
| Title | |
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| Authors | Yu J, Li X, Qi X, Ding Z, Su S, Yu L, Zhou L, Li Y |
| Journal | Scientific reports |
| Publication Date | 2024 May 29 |
| Abstract | Intramuscular fat (IMF) in pork holds significant importance for economic performance within the pig industry and dietary calcium supplementation enhances the accumulation of intramuscular fat. Additionally, calcium ions inhibit translation and reduce protein synthesis. However, the mechanism by which calcium regulates IMF deposition in muscle through translation remains largely unknown. In this study, we compared the ribosome profiles of the longissimus dorsi muscles of Duroc × Landrace × Large white pigs from the normal calcium (NC) group or calcium supplement (HC) group by Ribo-seq, and RNA-seq. By integrating multiple-omics analysis, we further discovered 437 genes that were transcriptionally unchanged but translationally altered and these genes were significantly enriched in the oxidative phosphorylation signaling pathway. Furthermore, experimental data showed that inhibiting the expression of COX10 and mtND4L increased triglyceride accumulation in C2C12 cells, providing new targets for intramuscular fat deposition. Finally, this work links dietary calcium, translation regulation and IMF deposition, providing a new strategy for both meat quality and economic performance within the pig industry. © 2024. The Author(s). |
| PMC | PMC11136974 |
| PMID | 38811812 |
| DOI |
| Run Accession | Study Accession | Scientific Name | Cell Line | Library Type | Treatment | GWIPS-viz | Trips-Viz | Reads | BAM | BigWig (F) | BigWig (R) | ||
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| SRR28770500 | PRJNA1103142 | Sus scrofa | 0.0 | Ribo-Seq | 0.0 |  |
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| SRR28770499 | PRJNA1103142 | Sus scrofa | 0.0 | Ribo-Seq | 0.0 | |
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| SRR28770498 | PRJNA1103142 | Sus scrofa | 0.0 | Ribo-Seq | 0.0 | |
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| SRR28770497 | PRJNA1103142 | Sus scrofa | 0.0 | Ribo-Seq | 0.0 | |
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| SRR28770496 | PRJNA1103142 | Sus scrofa | 0.0 | Ribo-Seq | 0.0 | |
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| SRR28770495 | PRJNA1103142 | Sus scrofa | 0.0 | Ribo-Seq | 0.0 | |
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| Run Accession | Study Accession | Scientific Name | Cell Line | Library Type | Treatment | GWIPS-viz | Trips-Viz | Reads | BAM | BigWig (F) | BigWig (R) |
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