Niu et al. 2015 (PRJNA213675)

General Details

Title m6A-dependent regulation of messenger RNA stability
Organism
Number of Samples 11
Release Date 2013/07/30 00:00
Sequencing Types
Protocol Details

Study Links

Repository Details

SRA SRP028325
ENA SRP028325
GEO GSE49339
BioProject PRJNA213675

Publication

Title
Authors Niu Y, Zhao X, Wu YS, Li MM, Wang XJ, Yang YG
Journal Genomics, proteomics & bioinformatics
Publication Date 2013 Feb
Abstract N(6)-methyl-adenosine (m(6)A) is one of the most common and abundant modifications on RNA molecules present in eukaryotes. However, the biological significance of m(6)A methylation remains largely unknown. Several independent lines of evidence suggest that the dynamic regulation of m(6)A may have a profound impact on gene expression regulation. The m(6)A modification is catalyzed by an unidentified methyltransferase complex containing at least one subunit methyltransferase like 3 (METTL3). m(6)A modification on messenger RNAs (mRNAs) mainly occurs in the exonic regions and 3'-untranslated region (3'-UTR) as revealed by high-throughput m(6)A-seq. One significant advance in m(6)A research is the recent discovery of the first two m(6)A RNA demethylases fat mass and obesity-associated (FTO) gene and ALKBH5, which catalyze m(6)A demethylation in an α-ketoglutarate (α-KG)- and Fe(2+)-dependent manner. Recent studies in model organisms demonstrate that METTL3, FTO and ALKBH5 play important roles in many biological processes, ranging from development and metabolism to fertility. Moreover, perturbation of activities of these enzymes leads to the disturbed expression of thousands of genes at the cellular level, implicating a regulatory role of m(6)A in RNA metabolism. Given the vital roles of DNA and histone methylations in epigenetic regulation of basic life processes in mammals, the dynamic and reversible chemical m(6)A modification on RNA may also serve as a novel epigenetic marker of profound biological significances. Copyright © 2013. Production and hosting by Elsevier Ltd.
PMC PMC4357660
PMID 23453015
DOI
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)
SRR944652 PRJNA213675 Homo sapiens HeLa RNA-Seq 0.0
SRR944653 PRJNA213675 Homo sapiens HeLa Ribo-Seq Cycloheximide
SRR944654 PRJNA213675 Homo sapiens HeLa RNA-Seq 0.0
SRR944655 PRJNA213675 Homo sapiens HeLa Ribo-Seq Cycloheximide
SRR944656 PRJNA213675 Homo sapiens HeLa RNA-Seq 0.0
SRR944657 PRJNA213675 Homo sapiens HeLa RNA-Seq 0.0
SRR944658 PRJNA213675 Homo sapiens HeLa RNA-Seq 0.0
SRR944659 PRJNA213675 Homo sapiens HeLa Ribo-Seq Cycloheximide
SRR944660 PRJNA213675 Homo sapiens HeLa RNA-Seq 0.0
SRR944661 PRJNA213675 Homo sapiens HeLa RNA-Seq 0.0
SRR944662 PRJNA213675 Homo sapiens HeLa Ribo-Seq Cycloheximide
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)

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