Guo et al. 2015 (PRJNA223608)

General Details

Title Expanded identification and characterization of mammalian circular RNAs
Organism
Number of Samples 3
Release Date 2013/10/23 00:00
Sequencing Types
Protocol Details

Study Links

Repository Details

SRA SRP031849
ENA SRP031849
GEO
BioProject PRJNA223608

Publication

Title
Authors Guo JU,Agarwal V,Guo H,Bartel DP
Journal Genome biology
Publication Date 2014 Jul 29
Abstract The recent reports of two circular RNAs (circRNAs) with strong potential to act as microRNA (miRNA) sponges suggest that circRNAs might play important roles in regulating gene expression. However, the global properties of circRNAs are not well understood. We developed a computational pipeline to identify circRNAs and quantify their relative abundance from RNA-seq data. Applying this pipeline to a large set of non-poly(A)-selected RNA-seq data from the ENCODE project, we annotated 7,112 human circRNAs that were estimated to comprise at least 10% of the transcripts accumulating from their loci. Most circRNAs are expressed in only a few cell types and at low abundance, but they are no more cell-type-specific than are mRNAs with similar overall expression levels. Although most circRNAs overlap protein-coding sequences, ribosome profiling provides no evidence for their translation. We also annotated 635 mouse circRNAs, and although 20% of them are orthologous to human circRNAs, the sequence conservation of these circRNA orthologs is no higher than that of their neighboring linear exons. The previously proposed miR-7 sponge, CDR1as, is one of only two circRNAs with more miRNA sites than expected by chance, with the next best miRNA-sponge candidate deriving from a gene encoding a primate-specific zinc-finger protein, ZNF91. Our results provide a new framework for future investigation of this intriguing topological isoform while raising doubts regarding a biological function of most circRNAs.
PMC PMC4165365
PMID 25070500
DOI
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)
SRR1598975 PRJNA223608 Homo sapiens U2OS Ribo-Seq Cycloheximide
SRR1598981 PRJNA223608 Homo sapiens U2OS Ribo-Seq Cycloheximide
SRR1598971 PRJNA223608 Homo sapiens U2OS Ribo-Seq Cycloheximide
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)

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