Datasets

Title	Widespread Co-translational RNA Decay Reveals Ribosome Dynamics
Organism
Number of Samples	6
Release Date	2014/11/08 00:00
Sequencing Types
Protocol Details

Title
Authors	Pelechano V,Wei W,Steinmetz LM
Journal	Cell
Publication Date	2015 Jun 4
Abstract	It is generally assumed that mRNAs undergoing translation are protected from decay. Here, we show that mRNAs are, in fact, co-translationally degraded. This is a widespread and conserved process affecting most genes, where 5'-3' transcript degradation follows the last translating ribosome, producing an in vivo ribosomal footprint. By sequencing the ends of 5' phosphorylated mRNA degradation intermediates, we obtain a genome-wide drug-free measurement of ribosome dynamics. We identify general translation termination pauses in both normal and stress conditions. In addition, we describe novel codon-specific ribosomal pausing sites in response to oxidative stress that are dependent on the RNase Rny1. Our approach is simple and straightforward and does not require the use of translational inhibitors or in vitro RNA footprinting that can alter ribosome protection patterns. Copyright © 2015 Elsevier Inc. All rights reserved.
PMC	PMC4461875
PMID	26046441
DOI

Pelechano et al. 2015 (PRJNA266705)