Dhungel et al. 2014 (PRJNA270712)
General Details
| Title | Parkinson's disease genes VPS35 and EIF4G1 interact genetically and converge on alpha-synuclein |
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| Organism | |
| Number of Samples | 4 |
| Release Date | 2014/12/18 00:00 |
| Sequencing Types | |
| Protocol Details |
Study Links
| GWIPS-viz | Trips-Viz |
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| Visit GWIPS-viz | Visit Trips-Viz |
Repository Details
| SRA | SRP051314 |
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| ENA | SRP051314 |
| GEO | |
| BioProject | PRJNA270712 |
Publication
| Title | |
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| Authors | Dhungel N,Eleuteri S,Li LB,Kramer NJ,Chartron JW,Spencer B,Kosberg K,Fields JA,Stafa K,Adame A,Lashuel H,Frydman J,Shen K,Masliah E,Gitler AD |
| Journal | Neuron |
| Publication Date | 2015 Jan 7 |
| Abstract | Parkinson's disease (PD) is a common neurodegenerative disorder. Functional interactions between some PD genes, like PINK1 and parkin, have been identified, but whether other ones interact remains elusive. Here we report an unexpected genetic interaction between two PD genes, VPS35 and EIF4G1. We provide evidence that EIF4G1 upregulation causes defects associated with protein misfolding. Expression of a sortilin protein rescues these defects, downstream of VPS35, suggesting a potential role for sortilins in PD. We also show interactions between VPS35, EIF4G1, and α-synuclein, a protein with a key role in PD. We extend our findings from yeast to an animal model and show that these interactions are conserved in neurons and in transgenic mice. Our studies reveal unexpected genetic and functional interactions between two seemingly unrelated PD genes and functionally connect them to α-synuclein pathobiology in yeast, worms, and mouse. Finally, we provide a resource of candidate PD genes for future interrogation. Copyright © 2015 Elsevier Inc. All rights reserved. |
| PMC | PMC4289081 |
| PMID | 25533483 |
| DOI |
| Run Accession | Study Accession | Scientific Name | Cell Line | Library Type | Treatment | GWIPS-viz | Trips-Viz | Reads | BAM | BigWig (F) | BigWig (R) | ||
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| SRR1722638 | PRJNA270712 | Saccharomyces cerevisiae | Ribo-Seq | Cycloheximide |  |
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| SRR1722639 | PRJNA270712 | Saccharomyces cerevisiae | Ribo-Seq | Cycloheximide | |
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| SRR1722640 | PRJNA270712 | Saccharomyces cerevisiae | Ribo-Seq | Cycloheximide | |
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| SRR1722641 | PRJNA270712 | Saccharomyces cerevisiae | Ribo-Seq | Cycloheximide | |
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| Run Accession | Study Accession | Scientific Name | Cell Line | Library Type | Treatment | GWIPS-viz | Trips-Viz | Reads | BAM | BigWig (F) | BigWig (R) |
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