Davari et al. 2017 (PRJNA325659)
General Details
Title | Rapid Genome-wide Recruitment of RNA Polymerase II Drives Transcription, Splicing and Translation Events during T Cell Responses |
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Organism | |
Number of Samples | 8 |
Release Date | 2016/06/14 00:00 |
Sequencing Types | |
Protocol Details |
Study Links
GWIPS-viz | Trips-Viz |
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Repository Details
SRA | SRP076551 |
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ENA | SRP076551 |
GEO | GSE83351 |
BioProject | PRJNA325659 |
Publication
Title | |
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Authors | Davari K,Lichti J,Gallus C,Greulich F,Uhlenhaut NH,Heinig M,Friedel CC,Glasmacher E |
Journal | Cell reports |
Publication Date | 2017 Apr 18 |
Abstract | Activation of immune cells results in rapid functional changes, but how such fast changes are accomplished remains enigmatic. By combining time courses of 4sU-seq, RNA-seq, ribosome profiling (RP), and RNA polymerase II (RNA Pol II) ChIP-seq during T cell activation, we illustrate genome-wide temporal dynamics for ∼10,000 genes. This approach reveals not only immediate-early and posttranscriptionally regulated genes but also coupled changes in transcription and translation for >90% of genes. Recruitment, rather than release of paused RNA Pol II, primarily mediates transcriptional changes. This coincides with a genome-wide temporary slowdown in cotranscriptional splicing, even for polyadenylated mRNAs that are localized at the chromatin. Subsequent splicing optimization correlates with increasing Ser-2 phosphorylation of the RNA Pol II carboxy-terminal domain (CTD) and activation of the positive transcription elongation factor (pTEFb). Thus, rapid de novo recruitment of RNA Pol II dictates the course of events during T cell activation, particularly transcription, splicing, and consequently translation. Copyright © 2017 The Authors. Published by Elsevier Inc. All rights reserved. |
PMC | |
PMID | 28423325 |
DOI |
Run Accession | Study Accession | Scientific Name | Cell Line | Library Type | Treatment | GWIPS-viz | Trips-Viz | Reads | BAM | BigWig (F) | BigWig (R) | ||
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SRR3666767 | PRJNA325659 | Mus musculus | Th1 effector cell (CD4+ T cell) | Ribo-Seq | |||||||||
SRR3666768 | PRJNA325659 | Mus musculus | Th1 effector cell (CD4+ T cell) | Ribo-Seq | |||||||||
SRR3666769 | PRJNA325659 | Mus musculus | Th1 effector cell (CD4+ T cell) | Ribo-Seq | |||||||||
SRR3666770 | PRJNA325659 | Mus musculus | Th1 effector cell (CD4+ T cell) | Ribo-Seq | |||||||||
SRR3666771 | PRJNA325659 | Mus musculus | Th1 effector cell (CD4+ T cell) | Ribo-Seq | |||||||||
SRR3666772 | PRJNA325659 | Mus musculus | Th1 effector cell (CD4+ T cell) | Ribo-Seq | |||||||||
SRR3666773 | PRJNA325659 | Mus musculus | Th1 effector cell (CD4+ T cell) | Ribo-Seq | |||||||||
SRR3666774 | PRJNA325659 | Mus musculus | Th1 effector cell (CD4+ T cell) | Ribo-Seq | |||||||||
Run Accession | Study Accession | Scientific Name | Cell Line | Library Type | Treatment | GWIPS-viz | Trips-Viz | Reads | BAM | BigWig (F) | BigWig (R) |
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