Arango et al. 2018 (PRJNA396698)

General Details

Title Acetylation of cytidine in messenger RNA
Organism
Number of Samples 12
Release Date 2017/08/01 00:00
Sequencing Types
Protocol Details

Study Links

Repository Details

SRA SRP114504
ENA SRP114504
GEO GSE102113
BioProject PRJNA396698

Publication

Title
Authors Arango D,Sturgill D,Alhusaini N,Dillman AA,Sweet TJ,Hanson G,Hosogane M,Sinclair WR,Nanan KK,Mandler MD,Fox SD,Zengeya TT,Andresson T,Meier JL,Coller J,Oberdoerffer S
Journal Cell
Publication Date 2018 Dec 13
Abstract Generation of the 'epitranscriptome' through post-transcriptional ribonucleoside modification embeds a layer of regulatory complexity into RNA structure and function. Here, we describe N4-acetylcytidine (ac4C) as an mRNA modification that is catalyzed by the acetyltransferase NAT10. Transcriptome-wide mapping of ac4C revealed discretely acetylated regions that were enriched within coding sequences. Ablation of NAT10 reduced ac4C detection at the mapped mRNA sites and was globally associated with target mRNA downregulation. Analysis of mRNA half-lives revealed a NAT10-dependent increase in stability in the cohort of acetylated mRNAs. mRNA acetylation was further demonstrated to enhance substrate translation in vitro and in vivo. Codon content analysis within ac4C peaks uncovered a biased representation of cytidine within wobble sites that was empirically determined to influence mRNA decoding efficiency. These findings expand the repertoire of mRNA modifications to include an acetylated residue and establish a role for ac4C in the regulation of mRNA translation. Published by Elsevier Inc.
PMC PMC6295233
PMID 30449621
DOI
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)
SRR5886312 PRJNA396698 Homo sapiens RNA-Seq
SRR5886313 PRJNA396698 Homo sapiens RNA-Seq
SRR5886314 PRJNA396698 Homo sapiens RNA-Seq
SRR5886315 PRJNA396698 Homo sapiens RNA-Seq
SRR5886316 PRJNA396698 Homo sapiens Ribo-Seq
SRR5886317 PRJNA396698 Homo sapiens Ribo-Seq
SRR5886318 PRJNA396698 Homo sapiens Ribo-Seq
SRR5886319 PRJNA396698 Homo sapiens Ribo-Seq
SRR5886320 PRJNA396698 Homo sapiens Ribo-Seq
SRR5886321 PRJNA396698 Homo sapiens Ribo-Seq
SRR5886322 PRJNA396698 Homo sapiens Ribo-Seq
SRR5886323 PRJNA396698 Homo sapiens Ribo-Seq
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)

ⓘ For more Information on the columns shown here see: About