Arango et al. 2018 (PRJNA396698)
General Details
Title | Acetylation of cytidine in messenger RNA |
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Organism | |
Number of Samples | 12 |
Release Date | 2017/08/01 00:00 |
Sequencing Types | |
Protocol Details |
Study Links
GWIPS-viz | Trips-Viz |
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Visit GWIPS-viz | Visit Trips-Viz |
Repository Details
SRA | SRP114504 |
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ENA | SRP114504 |
GEO | GSE102113 |
BioProject | PRJNA396698 |
Publication
Title | |
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Authors | Arango D,Sturgill D,Alhusaini N,Dillman AA,Sweet TJ,Hanson G,Hosogane M,Sinclair WR,Nanan KK,Mandler MD,Fox SD,Zengeya TT,Andresson T,Meier JL,Coller J,Oberdoerffer S |
Journal | Cell |
Publication Date | 2018 Dec 13 |
Abstract | Generation of the 'epitranscriptome' through post-transcriptional ribonucleoside modification embeds a layer of regulatory complexity into RNA structure and function. Here, we describe N4-acetylcytidine (ac4C) as an mRNA modification that is catalyzed by the acetyltransferase NAT10. Transcriptome-wide mapping of ac4C revealed discretely acetylated regions that were enriched within coding sequences. Ablation of NAT10 reduced ac4C detection at the mapped mRNA sites and was globally associated with target mRNA downregulation. Analysis of mRNA half-lives revealed a NAT10-dependent increase in stability in the cohort of acetylated mRNAs. mRNA acetylation was further demonstrated to enhance substrate translation in vitro and in vivo. Codon content analysis within ac4C peaks uncovered a biased representation of cytidine within wobble sites that was empirically determined to influence mRNA decoding efficiency. These findings expand the repertoire of mRNA modifications to include an acetylated residue and establish a role for ac4C in the regulation of mRNA translation. Published by Elsevier Inc. |
PMC | PMC6295233 |
PMID | 30449621 |
DOI |
Run Accession | Study Accession | Scientific Name | Cell Line | Library Type | Treatment | GWIPS-viz | Trips-Viz | Reads | BAM | BigWig (F) | BigWig (R) | ||
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SRR5886312 | PRJNA396698 | Homo sapiens | RNA-Seq | ||||||||||
SRR5886313 | PRJNA396698 | Homo sapiens | RNA-Seq | ||||||||||
SRR5886314 | PRJNA396698 | Homo sapiens | RNA-Seq | ||||||||||
SRR5886315 | PRJNA396698 | Homo sapiens | RNA-Seq | ||||||||||
SRR5886316 | PRJNA396698 | Homo sapiens | Ribo-Seq | ||||||||||
SRR5886317 | PRJNA396698 | Homo sapiens | Ribo-Seq | ||||||||||
SRR5886318 | PRJNA396698 | Homo sapiens | Ribo-Seq | ||||||||||
SRR5886319 | PRJNA396698 | Homo sapiens | Ribo-Seq | ||||||||||
SRR5886320 | PRJNA396698 | Homo sapiens | Ribo-Seq | ||||||||||
SRR5886321 | PRJNA396698 | Homo sapiens | Ribo-Seq | ||||||||||
SRR5886322 | PRJNA396698 | Homo sapiens | Ribo-Seq | ||||||||||
SRR5886323 | PRJNA396698 | Homo sapiens | Ribo-Seq | ||||||||||
Run Accession | Study Accession | Scientific Name | Cell Line | Library Type | Treatment | GWIPS-viz | Trips-Viz | Reads | BAM | BigWig (F) | BigWig (R) |
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