Li et al. 2022 (PRJNA633140)

General Details

Title eIF4A2 targets developmental potency and histone H3.3 transcripts for translational control of embryonic and cancer stemness [Ribo-seq]
Organism
Number of Samples 16
Release Date 2020/05/15 00:00
Sequencing Types
Protocol Details

Study Links

Repository Details

SRA SRP261884
ENA SRP261884
GEO GSE150676
BioProject PRJNA633140

Publication

Title
Authors Li D, Yang J, Huang X, Zhou H, Wang J
Journal Science advances
Publication Date 2022 Apr
Abstract Translational control has emerged as a fundamental regulatory layer of proteome complexity that governs cellular identity and functions. As initiation is the rate-limiting step of translation, we carried out an RNA interference screen for key translation initiation factors required to maintain embryonic stem cell (ESC) identity. We identified eukaryotic translation initiation factor 4A2 (eIF4A2) and defined its mechanistic action through ribosomal protein S26-independent and -dependent ribosomes in translation initiation activation of messenger RNAs (mRNAs) encoding pluripotency factors and the histone variant H3.3 with demonstrated roles in maintaining stem cell pluripotency. eIF4A2 also mediates translation initiation activation of Ddx6, which acts together with eIF4A2 to restrict the totipotent two-cell transcription program in ESCs through Zscan4 mRNA degradation and translation repression. Accordingly, knockdown of eIF4A2 disrupts ESC proteome, causing the loss of ESC identity. Collectively, we establish a translational paradigm of the protein synthesis of pluripotency transcription factors and epigenetic regulators imposed on their established roles in controlling pluripotency.
PMC PMC8967233
PMID 35353581
DOI
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)
SRR11798518 PRJNA633140 Mus musculus mESC Ribo-Seq 0.0
SRR11798519 PRJNA633140 Mus musculus mESC Ribo-Seq 0.0
SRR11798520 PRJNA633140 Mus musculus mESC Ribo-Seq 0.0
SRR11798521 PRJNA633140 Mus musculus mESC Ribo-Seq 0.0
SRR11798522 PRJNA633140 Mus musculus mESC Ribo-Seq 0.0
SRR11798523 PRJNA633140 Mus musculus mESC Ribo-Seq 0.0
SRR11798524 PRJNA633140 Mus musculus mESC Ribo-Seq 0.0
SRR11798525 PRJNA633140 Mus musculus mESC Ribo-Seq 0.0
SRR11798526 PRJNA633140 Mus musculus mESC Ribo-Seq 0.0
SRR11798527 PRJNA633140 Mus musculus mESC Ribo-Seq 0.0
SRR11798528 PRJNA633140 Mus musculus mESC Ribo-Seq 0.0
SRR11798529 PRJNA633140 Mus musculus mESC Ribo-Seq 0.0
SRR11798530 PRJNA633140 Mus musculus mESC Ribo-Seq 0.0
SRR11798531 PRJNA633140 Mus musculus mESC Ribo-Seq 0.0
SRR11798532 PRJNA633140 Mus musculus mESC Ribo-Seq 0.0
SRR11798533 PRJNA633140 Mus musculus mESC Ribo-Seq 0.0
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)

ⓘ For more Information on the columns shown here see: About