Datasets

Title	Upregulation of 5’-terminal oligopyrimidine mRNA translation upon loss of the tumor suppressor ARF
Organism
Number of Samples	6
Release Date	2020/08/24 00:00
Sequencing Types
Protocol Details

Title
Authors	Cottrell KA,Chiou RC,Weber JD
Journal	Scientific reports
Publication Date	2020 Dec 17
Abstract	Tumor cells require nominal increases in protein synthesis in order to maintain high proliferation rates. As such, tumor cells must acquire enhanced ribosome production. How the numerous mutations in tumor cells ultimately achieve this aberrant production is largely unknown. The gene encoding ARF is the most commonly deleted gene in human cancer. ARF plays a significant role in regulating ribosomal RNA synthesis and processing, ribosome export into the cytoplasm, and global protein synthesis. Utilizing ribosome profiling, we show that ARF is a major suppressor of 5'-terminal oligopyrimidine mRNA translation. Genes with increased translational efficiency following loss of ARF include many ribosomal proteins and translation factors. Knockout of p53 largely phenocopies ARF loss, with increased protein synthesis and expression of 5'-TOP encoded proteins. The 5'-TOP regulators eIF4G1 and LARP1 are upregulated in Arf- and p53-null cells.
PMC	PMC7747592
PMID	33335292
DOI

Cottrell et al. 2020 (PRJNA658965)