Galloway et al. 2021 (PRJNA672952)

General Details

Title RNMT regulates the activated CD4 T cell transcriptome and translatome [Ribo-Seq]
Organism
Number of Samples 24
Release Date 2020/10/28 00:00
Sequencing Types
Protocol Details

Study Links

Repository Details

SRA SRP289492
ENA SRP289492
GEO GSE160326
BioProject PRJNA672952

Publication

Title
Authors Galloway A,Kaskar A,Ditsova D,Atrih A,Yoshikawa H,Gomez-Moreira C,Suska O,Warminski M,Grzela R,Lamond AI,Darzynkiewicz E,Jemielity J,Cowling VH
Journal Nucleic acids research
Publication Date 2021 Jul 9
Abstract The m7G cap is ubiquitous on RNAPII-transcribed RNA and has fundamental roles in eukaryotic gene expression, however its in vivo role in mammals has remained unknown. Here, we identified the m7G cap methyltransferase, RNMT, as a key mediator of T cell activation, which specifically regulates ribosome production. During T cell activation, induction of mRNA expression and ribosome biogenesis drives metabolic reprogramming, rapid proliferation and differentiation generating effector populations. We report that RNMT is induced by T cell receptor (TCR) stimulation and co-ordinates the mRNA, snoRNA and rRNA production required for ribosome biogenesis. Using transcriptomic and proteomic analyses, we demonstrate that RNMT selectively regulates the expression of terminal polypyrimidine tract (TOP) mRNAs, targets of the m7G-cap binding protein LARP1. The expression of LARP1 targets and snoRNAs involved in ribosome biogenesis is selectively compromised in Rnmt cKO CD4 T cells resulting in decreased ribosome synthesis, reduced translation rates and proliferation failure. By enhancing ribosome abundance, upregulation of RNMT co-ordinates mRNA capping and processing with increased translational capacity during T cell activation. © The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research.
PMC PMC8266598
PMID 34125914
DOI
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)
SRR12925970 PRJNA672952 Mus musculus 20 hour activated CD4 T cells Ribo-Seq
SRR12925971 PRJNA672952 Mus musculus 20 hour activated CD4 T cells Ribo-Seq
SRR12925972 PRJNA672952 Mus musculus 20 hour activated CD4 T cells Ribo-Seq
SRR12925973 PRJNA672952 Mus musculus 20 hour activated CD4 T cells Ribo-Seq
SRR12925974 PRJNA672952 Mus musculus 20 hour activated CD4 T cells Ribo-Seq
SRR12925975 PRJNA672952 Mus musculus 20 hour activated CD4 T cells Ribo-Seq
SRR12925976 PRJNA672952 Mus musculus 20 hour activated CD4 T cells Ribo-Seq
SRR12925977 PRJNA672952 Mus musculus 20 hour activated CD4 T cells Ribo-Seq
SRR12925978 PRJNA672952 Mus musculus 20 hour activated CD4 T cells Ribo-Seq
SRR12925979 PRJNA672952 Mus musculus 20 hour activated CD4 T cells Ribo-Seq
SRR12925980 PRJNA672952 Mus musculus 20 hour activated CD4 T cells Ribo-Seq
SRR12925981 PRJNA672952 Mus musculus 20 hour activated CD4 T cells Ribo-Seq
SRR12925982 PRJNA672952 Mus musculus 20 hour activated CD4 T cells Ribo-Seq
SRR12925983 PRJNA672952 Mus musculus 20 hour activated CD4 T cells Ribo-Seq
SRR12925984 PRJNA672952 Mus musculus 20 hour activated CD4 T cells Ribo-Seq
SRR12925985 PRJNA672952 Mus musculus 20 hour activated CD4 T cells Ribo-Seq
SRR12925986 PRJNA672952 Mus musculus 20 hour activated CD4 T cells Ribo-Seq
SRR12925987 PRJNA672952 Mus musculus 20 hour activated CD4 T cells Ribo-Seq
SRR12925988 PRJNA672952 Mus musculus 20 hour activated CD4 T cells Ribo-Seq
SRR12925989 PRJNA672952 Mus musculus 20 hour activated CD4 T cells Ribo-Seq
SRR12925990 PRJNA672952 Mus musculus 20 hour activated CD4 T cells Ribo-Seq
SRR12925991 PRJNA672952 Mus musculus 20 hour activated CD4 T cells Ribo-Seq
SRR12925992 PRJNA672952 Mus musculus 20 hour activated CD4 T cells Ribo-Seq
SRR12925993 PRJNA672952 Mus musculus 20 hour activated CD4 T cells Ribo-Seq
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)

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