Dai et al. 2021 (PRJNA677870)
General Details
| Title | N7-methylguanosine tRNA modification enhances oncogenic mRNA translation and promotes intrahepatic cholangiocarcinoma progression |
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| Organism | |
| Number of Samples | 4 |
| Release Date | 2020/11/12 00:00 |
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| Protocol Details |
Study Links
| GWIPS-viz | Trips-Viz |
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Repository Details
| SRA | SRP292287 |
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| ENA | SRP292287 |
| GEO | |
| BioProject | PRJNA677870 |
Publication
| Title | |
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| Authors | Dai Z,Liu H,Liao J,Huang C,Ren X,Zhu W,Zhu S,Peng B,Li S,Lai J,Liang L,Xu L,Peng S,Lin S,Kuang M |
| Journal | Molecular cell |
| Publication Date | 2021 Aug 19 |
| Abstract | Cancer cells selectively promote translation of specific oncogenic transcripts to facilitate cancer survival and progression, but the underlying mechanisms are poorly understood. Here, we find that N 7 -methylguanosine (m 7 G) tRNA modification and its methyltransferase complex components, METTL1 and WDR4, are significantly upregulated in intrahepatic cholangiocarcinoma (ICC) and associated with poor prognosis. We further reveal the critical role of METTL1/WDR4 in promoting ICC cell survival and progression using loss- and gain-of-function assays in vitro and in vivo. Mechanistically, m 7 G tRNA modification selectively regulates the translation of oncogenic transcripts, including cell-cycle and epidermal growth factor receptor (EGFR) pathway genes, in m 7 G-tRNA-decoded codon-frequency-dependent mechanisms. Moreover, using overexpression and knockout mouse models, we demonstrate the crucial oncogenic function of Mettl1-mediated m 7 G tRNA modification in promoting ICC tumorigenesis and progression in vivo. Our study uncovers the important physiological function and mechanism of METTL1-mediated m 7 G tRNA modification in the regulation of oncogenic mRNA translation and cancer progression. Copyright © 2021 Elsevier Inc. All rights reserved. |
| PMC | |
| PMID | 34352206 |
| DOI |
| Run Accession | Study Accession | Scientific Name | Cell Line | Library Type | Treatment | GWIPS-viz | Trips-Viz | Reads | BAM | BigWig (F) | BigWig (R) | ||
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| SRR14824509 | PRJNA677870 | Homo sapiens | wild type |  |
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| SRR14824510 | PRJNA677870 | Homo sapiens | wild type | Ribo-Seq | |
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| SRR14824511 | PRJNA677870 | Homo sapiens | Mettl1 knockdown | |
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| SRR14824512 | PRJNA677870 | Homo sapiens | Mettl1 knockdown | Ribo-Seq | |
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| Run Accession | Study Accession | Scientific Name | Cell Line | Library Type | Treatment | GWIPS-viz | Trips-Viz | Reads | BAM | BigWig (F) | BigWig (R) |
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