Datasets

Title	Ribosome impairment regulates intestinal stem cell identity via ZAKɑ activation
Organism
Number of Samples	12
Release Date	2021/07/15 00:00
Sequencing Types
Protocol Details

Title
Authors	Silva J,Alkan F,Ramalho S,Snieckute G,Prekovic S,Garcia AK,Hernández-Pérez S,van der Kammen R,Barnum D,Hoekman L,Altelaar M,Zwart W,Suijkerbuijk SJE,Bekker-Jensen S,Faller WJ
Journal	Nature communications
Publication Date	2022 Aug 2
Abstract	The small intestine is a rapidly proliferating organ that is maintained by a small population of Lgr5-expressing intestinal stem cells (ISCs). However, several Lgr5-negative ISC populations have been identified, and this remarkable plasticity allows the intestine to rapidly respond to both the local environment and to damage. However, the mediators of such plasticity are still largely unknown. Using intestinal organoids and mouse models, we show that upon ribosome impairment (driven by Rptor deletion, amino acid starvation, or low dose cyclohexamide treatment) ISCs gain an Lgr5-negative, fetal-like identity. This is accompanied by a rewiring of metabolism. Our findings suggest that the ribosome can act as a sensor of nutrient availability, allowing ISCs to respond to the local nutrient environment. Mechanistically, we show that this phenotype requires the activation of ZAKɑ, which in turn activates YAP, via SRC. Together, our data reveals a central role for ribosome dynamics in intestinal stem cells, and identify the activation of ZAKɑ as a critical mediator of stem cell identity. © 2022. The Author(s).
PMC	PMC9345940
PMID	35918345
DOI

Silva et al. 2022 (PRJNA747022)