Zhou et al. 2022 (PRJNA769267)

General Details

Title The dynamic rRNA ribomethylome drives stemness in acute myeloid leukemia [ribosome profiling]
Organism
Number of Samples 4
Release Date 2021/10/07 00:00
Sequencing Types
Protocol Details

Study Links

Repository Details

SRA SRP340351
ENA SRP340351
GEO GSE185489
BioProject PRJNA769267

Publication

Title
Authors Zhou F, Aroua N, Liu Y, Rohde C, Cheng J, Wirth AK, Fijalkowska D, Göllner S, Lotze M, Yun H, Yu X, Pabst C, Sauer T, Oellerich T, Serve H, Röllig C, Bornhäuser M, Thiede C, Baldus C, Frye M, Raffel S, Krijgsveld J, Jeremias I, Beckmann R, Trumpp A, Müller-Tidow C
Journal Cancer discovery
Publication Date 2023 Feb 6
Abstract The development and regulation of malignant self-renewal remain unresolved issues. Here, we provide biochemical, genetic, and functional evidence that dynamics in ribosomal RNA (rRNA) 2'-O-methylation regulate leukemia stem cell (LSC) activity in vivo. A comprehensive analysis of the rRNA 2'-O-methylation landscape of 94 patients with acute myeloid leukemia (AML) revealed dynamic 2'-O-methylation specifically at exterior sites of ribosomes. The rRNA 2'-O-methylation pattern is closely associated with AML development stage and LSC gene expression signature. Forced expression of the 2'-O-methyltransferase fibrillarin (FBL) induced an AML stem cell phenotype and enabled engraftment of non-LSC leukemia cells in NSG mice. Enhanced 2'-O-methylation redirected the ribosome translation program toward amino acid transporter mRNAs enriched in optimal codons and subsequently increased intracellular amino acid levels. Methylation at the single site 18S-guanosine 1447 was instrumental for LSC activity. Collectively, our work demonstrates that dynamic 2'-O-methylation at specific sites on rRNAs shifts translational preferences and controls AML LSC self-renewal. We establish the complete rRNA 2'-O-methylation landscape in human AML. Plasticity of rRNA 2'-O-methylation shifts protein translation toward an LSC phenotype. This dynamic process constitutes a novel concept of how cancers reprogram cell fate and function. This article is highlighted in the In This Issue feature, p. 247. ©2022 The Authors; Published by the American Association for Cancer Research.
PMC PMC9900322
PMID 36259929
DOI
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)
SRR16230267 PRJNA769267 Homo sapiens 0.0 0.0 0.0
SRR16230268 PRJNA769267 Homo sapiens 0.0 0.0 0.0
SRR16230269 PRJNA769267 Homo sapiens 0.0 0.0 0.0
SRR16230270 PRJNA769267 Homo sapiens 0.0 0.0 0.0
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)

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