Vijjamarri et al. 2023 (PRJNA932446)
General Details
| Title | mRNA decapping activators Pat1 and Dhh1 regulate transcript abundance and translation to tune cellular responses to nutrient availability |
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| Organism | |
| Number of Samples | 9 |
| Release Date | 2023/02/07 00:00 |
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Study Links
| GWIPS-viz | Trips-Viz |
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Repository Details
| SRA | SRP421393 |
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| ENA | SRP421393 |
| GEO | |
| BioProject | PRJNA932446 |
Publication
| Title | |
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| Authors | Vijjamarri AK,Gupta N,Onu C,Niu X,Zhang F,Kumar R,Lin Z,Greenberg ML,Hinnebusch AG |
| Journal | Nucleic acids research |
| Publication Date | 2023 Sep 22 |
| Abstract | We have examined the roles of yeast mRNA decapping-activators Pat1 and Dhh1 in repressing the translation and abundance of specific mRNAs in nutrient-replete cells using ribosome profiling, RNA-Seq, CAGE analysis of capped mRNAs, RNA Polymerase II ChIP-Seq, and TMT-mass spectrometry of mutants lacking one or both factors. Although the Environmental Stress Response (ESR) is activated in dhh1Δ and pat1Δ mutants, hundreds of non-ESR transcripts are elevated in a manner indicating cumulative repression by Pat1 and Dhh1 in wild-type cells. These mRNAs show both reduced decapping and diminished transcription in the mutants, indicating that impaired mRNA turnover drives transcript derepression in cells lacking Dhh1 or Pat1. mRNA degradation stimulated by Dhh1/Pat1 is not dictated by poor translation nor enrichment for suboptimal codons. Pat1 and Dhh1 also collaborate to reduce translation and protein production from many mRNAs. Transcripts showing concerted translational repression by Pat1/Dhh1 include mRNAs involved in cell adhesion or utilization of the poor nitrogen source allantoin. Pat1/Dhh1 also repress numerous transcripts involved in respiration, catabolism of non-preferred carbon or nitrogen sources, or autophagy; and we obtained evidence for elevated respiration and autophagy in the mutants. Thus, Pat1 and Dhh1 function as post-transcriptional repressors of multiple pathways normally activated only during nutrient limitation. Published by Oxford University Press on behalf of Nucleic Acids Research 2023. |
| PMC | PMC10516646 |
| PMID | 37439347 |
| DOI |
| Run Accession | Study Accession | Scientific Name | Cell Line | Library Type | Treatment | GWIPS-viz | Trips-Viz | Reads | BAM | BigWig (F) | BigWig (R) | ||
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| SRR23363979 | PRJNA932446 | Saccharomyces cerevisiae | yeast cells | Ribo-Seq |  |
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| SRR23363978 | PRJNA932446 | Saccharomyces cerevisiae | yeast cells | Ribo-Seq | |
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| SRR23363977 | PRJNA932446 | Saccharomyces cerevisiae | yeast cells | Ribo-Seq | |
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| SRR23363976 | PRJNA932446 | Saccharomyces cerevisiae | yeast cells | Ribo-Seq | |
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| SRR23363975 | PRJNA932446 | Saccharomyces cerevisiae | yeast cells | Ribo-Seq | |
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| SRR23363974 | PRJNA932446 | Saccharomyces cerevisiae | yeast cells | Ribo-Seq | |
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| SRR23363973 | PRJNA932446 | Saccharomyces cerevisiae | yeast cells | Ribo-Seq | |
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| SRR23363972 | PRJNA932446 | Saccharomyces cerevisiae | yeast cells | Ribo-Seq | |
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| SRR23363971 | PRJNA932446 | Saccharomyces cerevisiae | yeast cells | Ribo-Seq | |
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| Run Accession | Study Accession | Scientific Name | Cell Line | Library Type | Treatment | GWIPS-viz | Trips-Viz | Reads | BAM | BigWig (F) | BigWig (R) |
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