Diamond et al. 2023 (PRJNA967640)
General Details
| Title | Dysregulation of amino acid metabolism upon rapid depletion of cap-binding protein eIF4E [RNA-Seq] |
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| Organism | |
| Number of Samples | 8 |
| Release Date | 2023/05/05 00:00 |
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| Protocol Details |
Study Links
| GWIPS-viz | Trips-Viz |
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Repository Details
| SRA | SRP436183 |
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| ENA | SRP436183 |
| GEO | GSE231758 |
| BioProject | PRJNA967640 |
Publication
| Title | |
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| Authors | Diamond PD,McGlincy NJ,Ingolia NT |
| Journal | bioRxiv : the preprint server for biology |
| Publication Date | 2023 May 12 |
| Abstract | Protein synthesis is a crucial but metabolically costly biological process that must be tightly coordinated with cellular needs and nutrient availability. In response to environmental stress, translation initiation is modulated to control protein output while meeting new demands. The cap-binding protein eIF4E-the earliest contact between mRNAs and the translation machinery-serves as one point of control, but its contributions to mRNA-specific translation regulation remain poorly understood. To survey eIF4E-dependent translational control, we acutely depleted eIF4E and determined how this impacts protein synthesis. Despite its essentiality, eIF4E depletion had surprisingly modest effects on cell growth and protein synthesis. Analysis of transcript-level changes revealed that long-lived transcripts were downregulated, likely reflecting accelerated turnover. Paradoxically, eIF4E depletion led to simultaneous upregulation of genes involved in catabolism of aromatic amino acids, which arose as secondary effects of reduced protein biosynthesis on amino acid pools, and genes involved in the biosynthesis of amino acids. These futile cycles of amino acid synthesis and degradation were driven, in part, by translational activation of GCN4 , a transcription factor typically induced by amino acid starvation. Furthermore, we identified a novel regulatory mechanism governing translation of PCL5 , a negative regulator of Gcn4, that provides a consistent protein-to-mRNA ratio under varied translation environments. This translational control was partial dependent on a uniquely long poly-(A) tract in the PCL5 5' UTR and on poly-(A) binding protein. Collectively, these results highlight how eIF4E connects translation to amino acid homeostasis and stress responses and uncovers new mechanisms underlying how cells tightly control protein synthesis during environmental challenges. |
| PMC | PMC10197679 |
| PMID | 37214807 |
| DOI |
| Run Accession | Study Accession | Scientific Name | Cell Line | Library Type | Treatment | GWIPS-viz | Trips-Viz | Reads | BAM | BigWig (F) | BigWig (R) | ||
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| SRR24451169 | PRJNA967640 | Saccharomyces cerevisiae | BY4742 | Ribo-Seq |  |
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| SRR24451168 | PRJNA967640 | Saccharomyces cerevisiae | BY4742 | Ribo-Seq | |
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| SRR24451167 | PRJNA967640 | Saccharomyces cerevisiae | BY4742 | Ribo-Seq | |
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| SRR24451166 | PRJNA967640 | Saccharomyces cerevisiae | BY4742 | Ribo-Seq | |
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| SRR24451161 | PRJNA967640 | Saccharomyces cerevisiae | BY4742 | Ribo-Seq | |
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| SRR24451160 | PRJNA967640 | Saccharomyces cerevisiae | BY4742 | Ribo-Seq | |
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| SRR24451159 | PRJNA967640 | Saccharomyces cerevisiae | BY4742 | Ribo-Seq | |
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| SRR24451158 | PRJNA967640 | Saccharomyces cerevisiae | BY4742 | Ribo-Seq | |
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| Run Accession | Study Accession | Scientific Name | Cell Line | Library Type | Treatment | GWIPS-viz | Trips-Viz | Reads | BAM | BigWig (F) | BigWig (R) |
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