Eichhorn et al. 2015 (PRJNA258219)

General Details

Title mRNA destabilization is the dominant effect of mammalian microRNAs by the time substantial repression ensues (sequencing)
Organism
Number of Samples 54
Release Date 2014/08/14 00:00
Sequencing Types
Protocol Details

Study Links

Repository Details

SRA SRP045501
ENA SRP045501
GEO GSE60426
BioProject PRJNA258219

Publication

Title
Authors Eichhorn SW,Guo H,McGeary SE,Rodriguez-Mias RA,Shin C,Baek D,Hsu SH,Ghoshal K,Villén J,Bartel DP
Journal Molecular cell
Publication Date 2014 Oct 2
Abstract MicroRNAs (miRNAs) regulate target mRNAs through a combination of translational repression and mRNA destabilization, with mRNA destabilization dominating at steady state in the few contexts examined globally. Here, we extend the global steady-state measurements to additional mammalian contexts and find that regardless of the miRNA, cell type, growth condition, or translational state, mRNA destabilization explains most (66%->90%) miRNA-mediated repression. We also determine the relative dynamics of translational repression and mRNA destabilization for endogenous mRNAs as a miRNA is induced. Although translational repression occurs rapidly, its effect is relatively weak, such that by the time consequential repression ensues, the effect of mRNA destabilization dominates. These results imply that consequential miRNA-mediated repression is largely irreversible and provide other insights into the nature of miRNA-mediated regulation. They also simplify future studies, dramatically extending the known contexts and time points for which monitoring mRNA changes captures most of the direct miRNA effects. Copyright © 2014 Elsevier Inc. All rights reserved.
PMC PMC4292926
PMID 25263593
DOI
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)
SRR1551122 PRJNA258219 Mus musculus primary B cell Ribo-Seq
SRR1551123 PRJNA258219 Mus musculus primary B cell Ribo-Seq
SRR1551124 PRJNA258219 Mus musculus primary B cell Ribo-Seq
SRR1551125 PRJNA258219 Mus musculus primary B cell Ribo-Seq
SRR1551126 PRJNA258219 Mus musculus primary B cell Ribo-Seq
SRR1551127 PRJNA258219 Mus musculus primary B cell Ribo-Seq
SRR1551128 PRJNA258219 Mus musculus primary B cell Ribo-Seq
SRR1551129 PRJNA258219 Mus musculus primary B cell Ribo-Seq
SRR1551130 PRJNA258219 Mus musculus primary B cell Ribo-Seq
SRR1551131 PRJNA258219 Mus musculus U2OS Ribo-Seq
SRR1551132 PRJNA258219 Mus musculus U2OS Ribo-Seq
SRR1551140 PRJNA258219 Mus musculus U2OS Ribo-Seq
SRR1551141 PRJNA258219 Mus musculus U2OS Ribo-Seq
SRR1551142 PRJNA258219 Mus musculus U2OS Ribo-Seq
SRR1551143 PRJNA258219 Mus musculus Ribo-Seq
SRR1551144 PRJNA258219 Mus musculus Ribo-Seq
SRR1551145 PRJNA258219 Mus musculus Ribo-Seq
SRR1551146 PRJNA258219 Mus musculus Ribo-Seq
SRR1551147 PRJNA258219 Mus musculus Ribo-Seq
SRR1551148 PRJNA258219 Mus musculus Ribo-Seq
SRR1551149 PRJNA258219 Mus musculus Ribo-Seq
SRR1551150 PRJNA258219 Mus musculus Ribo-Seq
SRR1551151 PRJNA258219 Mus musculus 3T3 Ribo-Seq
SRR1551155 PRJNA258219 Homo sapiens 3T3 Ribo-Seq
SRR1551159 PRJNA258219 Homo sapiens 3T3 Ribo-Seq
SRR1551163 PRJNA258219 Homo sapiens 3T3 Ribo-Seq
SRR1551165 PRJNA258219 Homo sapiens 3T3 Ribo-Seq Cycloheximide
SRR1551167 PRJNA258219 Homo sapiens 3T3 Ribo-Seq
SRR1551169 PRJNA258219 Mus musculus 3T3 Ribo-Seq
SRR1593031 PRJNA258219 Mus musculus 3T3 Ribo-Seq
SRR1551171 PRJNA258219 Mus musculus 3T3 Ribo-Seq
SRR1551177 PRJNA258219 Mus musculus 3T3 Ribo-Seq
SRR1551178 PRJNA258219 Mus musculus 3T3 Ribo-Seq
SRR1551179 PRJNA258219 Mus musculus 3T3 Ribo-Seq
SRR1551180 PRJNA258219 Mus musculus 3T3 Ribo-Seq
SRR1551181 PRJNA258219 Mus musculus 3T3 Ribo-Seq
SRR1551186 PRJNA258219 Mus musculus 3T3 Ribo-Seq
SRR1551187 PRJNA258219 Mus musculus 3T3 Ribo-Seq
SRR1551188 PRJNA258219 Mus musculus 3T3 Ribo-Seq
SRR1551189 PRJNA258219 Mus musculus 3T3 Ribo-Seq
SRR1551196 PRJNA258219 Mus musculus 3T3 Ribo-Seq
SRR1551197 PRJNA258219 Mus musculus 3T3 Ribo-Seq
SRR1551198 PRJNA258219 Mus musculus 3T3 Ribo-Seq
SRR1551199 PRJNA258219 Mus musculus 3T3 Ribo-Seq
SRR1551200 PRJNA258219 Mus musculus 3T3 Ribo-Seq
SRR1551201 PRJNA258219 Mus musculus 3T3 Ribo-Seq
SRR1551204 PRJNA258219 Mus musculus 3T3 Ribo-Seq
SRR1551205 PRJNA258219 Mus musculus 3T3 Ribo-Seq
SRR1551208 PRJNA258219 Mus musculus 3T3 Ribo-Seq
SRR1551209 PRJNA258219 Mus musculus 3T3 Ribo-Seq
SRR1551212 PRJNA258219 Mus musculus 3T3 Ribo-Seq
SRR1551213 PRJNA258219 Mus musculus U2OS Ribo-Seq
SRR1551216 PRJNA258219 Mus musculus U2OS Ribo-Seq
SRR1551217 PRJNA258219 Mus musculus U2OS Ribo-Seq
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)

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