Datasets

Title	Heterogeneous ribosomes exist and selectively translate distinct subpools of mRNAs in stem cells
Organism
Number of Samples	12
Release Date	2015/09/23 00:00
Sequencing Types
Protocol Details

Title
Authors	Shi Z,Fujii K,Kovary KM,Genuth NR,Röst HL,Teruel MN,Barna M
Journal	Molecular cell
Publication Date	2017 Jul 6
Abstract	Emerging studies have linked the ribosome to more selective control of gene regulation. However, an outstanding question is whether ribosome heterogeneity at the level of core ribosomal proteins (RPs) exists and enables ribosomes to preferentially translate specific mRNAs genome-wide. Here, we measured the absolute abundance of RPs in translating ribosomes and profiled transcripts that are enriched or depleted from select subsets of ribosomes within embryonic stem cells. We find that heterogeneity in RP composition endows ribosomes with differential selectivity for translating subpools of transcripts, including those controlling metabolism, cell cycle, and development. As an example, mRNAs enriched in binding to RPL10A/uL1-containing ribosomes are shown to require RPL10A/uL1 for their efficient translation. Within several of these transcripts, this level of regulation is mediated, at least in part, by internal ribosome entry sites. Together, these results reveal a critical functional link between ribosome heterogeneity and the post-transcriptional circuitry of gene expression. Copyright © 2017 Elsevier Inc. All rights reserved.
PMC	PMC5548184
PMID	28625553
DOI

Shi et al. 2017 (PRJNA297039)