Allmeroth et al. 2021 (PRJNA692108)
General Details
| Title | Effect of N1-acetylspermidine treatment on the translatome of hair follicle stem cell organoid cultures |
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| Organism | |
| Number of Samples | 8 |
| Release Date | 2021/01/14 00:00 |
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| Protocol Details |
Study Links
| GWIPS-viz | Trips-Viz |
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Repository Details
| SRA | SRP301728 |
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| ENA | SRP301728 |
| GEO | GSE164806 |
| BioProject | PRJNA692108 |
Publication
| Title | |
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| Authors | Allmeroth K, Kim CS, Annibal A, Pouikli A, Koester J, Derisbourg MJ, Andrés Chacón-Martínez C, Latza C, Antebi A, Tessarz P, Wickström SA, Denzel MS |
| Journal | Journal of cell science |
| Publication Date | 2021 May 1 |
| Abstract | Stem cell differentiation is accompanied by increased mRNA translation. The rate of protein biosynthesis is influenced by the polyamines putrescine, spermidine and spermine, which are essential for cell growth and stem cell maintenance. However, the role of polyamines as endogenous effectors of stem cell fate and whether they act through translational control remains obscure. Here, we investigate the function of polyamines in stem cell fate decisions using hair follicle stem cell (HFSC) organoids. Compared to progenitor cells, HFSCs showed lower translation rates, correlating with reduced polyamine levels. Surprisingly, overall polyamine depletion decreased translation but did not affect cell fate. In contrast, specific depletion of natural polyamines mediated by spermidine/spermine N1-acetyltransferase (SSAT; also known as SAT1) activation did not reduce translation but enhanced stemness. These results suggest a translation-independent role of polyamines in cell fate regulation. Indeed, we identified N1-acetylspermidine as a determinant of cell fate that acted through increasing self-renewal, and observed elevated N1-acetylspermidine levels upon depilation-mediated HFSC proliferation and differentiation in vivo. Overall, this study delineates the diverse routes of polyamine metabolism-mediated regulation of stem cell fate decisions. This article has an associated First Person interview with the first author of the paper. © 2021. Published by The Company of Biologists Ltd. |
| PMC | PMC8182411 |
| PMID | 33973637 |
| DOI |
| Run Accession | Study Accession | Scientific Name | Cell Line | Library Type | Treatment | GWIPS-viz | Trips-Viz | Reads | BAM | BigWig (F) | BigWig (R) | ||
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| SRR13435518 | PRJNA692108 | Mus musculus | NA_HFSC organoid culture | Ribo-Seq | 0.0 |  |
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| SRR13435519 | PRJNA692108 | Mus musculus | NA_HFSC organoid culture | Ribo-Seq | 0.0 | |
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| SRR13435520 | PRJNA692108 | Mus musculus | NA_HFSC organoid culture | Ribo-Seq | 0.0 | |
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| SRR13435521 | PRJNA692108 | Mus musculus | NA_HFSC organoid culture | Ribo-Seq | 0.0 | |
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| SRR13435522 | PRJNA692108 | Mus musculus | NA_HFSC organoid culture | Ribo-Seq | 0.0 | |
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| SRR13435523 | PRJNA692108 | Mus musculus | NA_HFSC organoid culture | Ribo-Seq | 0.0 | |
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| SRR13435524 | PRJNA692108 | Mus musculus | NA_HFSC organoid culture | Ribo-Seq | 0.0 | |
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| SRR13435525 | PRJNA692108 | Mus musculus | NA_HFSC organoid culture | Ribo-Seq | 0.0 | |
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| Run Accession | Study Accession | Scientific Name | Cell Line | Library Type | Treatment | GWIPS-viz | Trips-Viz | Reads | BAM | BigWig (F) | BigWig (R) |
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