Datasets

Title	Transcription-wide distribution of dihydrouridine (D) into mRNAs reveals its requirement for meiotic chromosome segregation [Ribo/RNA-seq]
Organism
Number of Samples	6
Release Date	2021/08/10 00:00
Sequencing Types
Protocol Details

Title
Authors	Finet O,Yague-Sanz C,Krüger LK,Tran P,Migeot V,Louski M,Nevers A,Rougemaille M,Sun J,Ernst FGM,Wacheul L,Wery M,Morillon A,Dedon P,Lafontaine DLJ,Hermand D
Journal	Molecular cell
Publication Date	2022 Jan 20
Abstract	The epitranscriptome has emerged as a new fundamental layer of control of gene expression. Nevertheless, the determination of the transcriptome-wide occupancy and function of RNA modifications remains challenging. Here we have developed Rho-seq, an integrated pipeline detecting a range of modifications through differential modification-dependent rhodamine labeling. Using Rho-seq, we confirm that the reduction of uridine to dihydrouridine (D) by the Dus reductase enzymes targets tRNAs in E. coli and fission yeast. We find that the D modification is also present on fission yeast mRNAs, particularly those encoding cytoskeleton-related proteins, which is supported by large-scale proteome analyses and ribosome profiling. We show that the α-tubulin encoding mRNA nda2 undergoes Dus3-dependent dihydrouridylation, which affects its translation. The absence of the modification on nda2 mRNA strongly impacts meiotic chromosome segregation, resulting in low gamete viability. Applying Rho-seq to human cells revealed that tubulin mRNA dihydrouridylation is evolutionarily conserved. Copyright © 2021 Elsevier Inc. All rights reserved.
PMC	PMC8792297
PMID	34798057
DOI

Finet et al. 2021 (PRJNA753526)