Litsios et al. 2024 (PRJNA1003962)

General Details

Title Proteome-scale movements and compartment connectivity during the eukaryotic cell cycle
Organism
Number of Samples 24
Release Date 2023/08/09 00:00
Sequencing Types
Protocol Details

Study Links

Repository Details

SRA SRP454359
ENA SRP454359
GEO 0.0
BioProject PRJNA1003962

Publication

Title
Authors Litsios A, Grys BT, Kraus OZ, Friesen H, Ross C, Masinas MPD, Forster DT, Couvillion MT, Timmermann S, Billmann M, Myers C, Johnsson N, Churchman LS, Boone C, Andrews BJ
Journal Cell
Publication Date 2024 Mar 14
Abstract Cell cycle progression relies on coordinated changes in the composition and subcellular localization of the proteome. By applying two distinct convolutional neural networks on images of millions of live yeast cells, we resolved proteome-level dynamics in both concentration and localization during the cell cycle, with resolution of ∼20 subcellular localization classes. We show that a quarter of the proteome displays cell cycle periodicity, with proteins tending to be controlled either at the level of localization or concentration, but not both. Distinct levels of protein regulation are preferentially utilized for different aspects of the cell cycle, with changes in protein concentration being mostly involved in cell cycle control and changes in protein localization in the biophysical implementation of the cell cycle program. We present a resource for exploring global proteome dynamics during the cell cycle, which will aid in understanding a fundamental biological process at a systems level. Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved.
PMC PMC10947830
PMID 38452761
DOI
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)
SRR25592737 PRJNA1003962 Saccharomyces cerevisiae U2OS Ribo-Seq 0.0
SRR25592736 PRJNA1003962 Saccharomyces cerevisiae U2OS Ribo-Seq 0.0
SRR25592735 PRJNA1003962 Saccharomyces cerevisiae U2OS Ribo-Seq 0.0
SRR25592734 PRJNA1003962 Saccharomyces cerevisiae U2OS Ribo-Seq 0.0
SRR25592733 PRJNA1003962 Saccharomyces cerevisiae U2OS Ribo-Seq 0.0
SRR25592732 PRJNA1003962 Saccharomyces cerevisiae U2OS Ribo-Seq 0.0
SRR25592731 PRJNA1003962 Saccharomyces cerevisiae U2OS Ribo-Seq 0.0
SRR25592730 PRJNA1003962 Saccharomyces cerevisiae U2OS Ribo-Seq 0.0
SRR25592729 PRJNA1003962 Saccharomyces cerevisiae U2OS Ribo-Seq 0.0
SRR25592728 PRJNA1003962 Saccharomyces cerevisiae U2OS Ribo-Seq 0.0
SRR25592727 PRJNA1003962 Saccharomyces cerevisiae U2OS Ribo-Seq 0.0
SRR25592726 PRJNA1003962 Saccharomyces cerevisiae U2OS Ribo-Seq 0.0
SRR25592725 PRJNA1003962 Saccharomyces cerevisiae U2OS RNA-Seq 0.0
SRR25592724 PRJNA1003962 Saccharomyces cerevisiae U2OS RNA-Seq 0.0
SRR25592723 PRJNA1003962 Saccharomyces cerevisiae U2OS RNA-Seq 0.0
SRR25592722 PRJNA1003962 Saccharomyces cerevisiae U2OS RNA-Seq 0.0
SRR25592721 PRJNA1003962 Saccharomyces cerevisiae U2OS RNA-Seq 0.0
SRR25592720 PRJNA1003962 Saccharomyces cerevisiae U2OS RNA-Seq 0.0
SRR25592719 PRJNA1003962 Saccharomyces cerevisiae U2OS RNA-Seq 0.0
SRR25592718 PRJNA1003962 Saccharomyces cerevisiae U2OS RNA-Seq 0.0
SRR25592717 PRJNA1003962 Saccharomyces cerevisiae U2OS RNA-Seq 0.0
SRR25592716 PRJNA1003962 Saccharomyces cerevisiae U2OS RNA-Seq 0.0
SRR25592715 PRJNA1003962 Saccharomyces cerevisiae U2OS RNA-Seq 0.0
SRR25592714 PRJNA1003962 Saccharomyces cerevisiae U2OS RNA-Seq 0.0
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)

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