Oh et al. 2011 (PRJNA148631)

General Details

Title Selective ribosome profiling reveals the co-translational chaperone action of trigger factor in vivo
Organism
Number of Samples 8
Release Date 2011/12/09 00:00
Sequencing Types
Protocol Details

Study Links

Repository Details

SRA SRP009321
ENA SRP009321
GEO GSE33671
BioProject PRJNA148631

Publication

Title
Authors Oh E,Becker AH,Sandikci A,Huber D,Chaba R,Gloge F,Nichols RJ,Typas A,Gross CA,Kramer G,Weissman JS,Bukau B
Journal Cell
Publication Date 2011 Dec 9
Abstract As nascent polypeptides exit ribosomes, they are engaged by a series of processing, targeting, and folding factors. Here, we present a selective ribosome profiling strategy that enables global monitoring of when these factors engage polypeptides in the complex cellular environment. Studies of the Escherichia coli chaperone trigger factor (TF) reveal that, though TF can interact with many polypeptides, β-barrel outer-membrane proteins are the most prominent substrates. Loss of TF leads to broad outer-membrane defects and premature, cotranslational protein translocation. Whereas in vitro studies suggested that TF is prebound to ribosomes waiting for polypeptides to emerge from the exit channel, we find that in vivo TF engages ribosomes only after ~100 amino acids are translated. Moreover, excess TF interferes with cotranslational removal of the N-terminal formyl methionine. Our studies support a triaging model in which proper protein biogenesis relies on the fine-tuned, sequential engagement of processing, targeting, and folding factors. Copyright © 2011 Elsevier Inc. All rights reserved.
PMC PMC3277850
PMID 22153074
DOI
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)
SRR364363 PRJNA148631 Escherichia coli Ribo-Seq
SRR364364 PRJNA148631 Escherichia coli Ribo-Seq
SRR364365 PRJNA148631 Escherichia coli Ribo-Seq
SRR364366 PRJNA148631 Escherichia coli Ribo-Seq
SRR364367 PRJNA148631 Escherichia coli Ribo-Seq
SRR364368 PRJNA148631 Escherichia coli Ribo-Seq
SRR364369 PRJNA148631 Escherichia coli Ribo-Seq
SRR364370 PRJNA148631 Escherichia coli Ribo-Seq
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)

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