Datasets

Title	Sequence selectivity of macrolide-induced translational regulation
Organism
Number of Samples	14
Release Date	2014/04/29 00:00
Sequencing Types
Protocol Details

Title
Authors	Davis AR,Gohara DW,Yap MN
Journal	Proceedings of the National Academy of Sciences of the United States of America
Publication Date	2014 Oct 28
Abstract	The prevailing 'plug-in-the-bottle' model suggests that macrolide antibiotics inhibit translation by binding inside the ribosome tunnel and indiscriminately arresting the elongation of every nascent polypeptide after the synthesis of six to eight amino acids. To test this model, we performed a genome-wide analysis of translation in azithromycin-treated Staphylococcus aureus. In contrast to earlier predictions, we found that the macrolide does not preferentially induce ribosome stalling near the 5' end of mRNAs, but rather acts at specific stalling sites that are scattered throughout the entire coding region. These sites are highly enriched in prolines and charged residues and are strikingly similar to other ligand-independent ribosome stalling motifs. Interestingly, the addition of structurally related macrolides had dramatically different effects on stalling efficiency. Our data suggest that ribosome stalling can occur at a surprisingly large number of low-complexity motifs in a fashion that depends only on a few arrest-inducing residues and the presence of a small molecule inducer.
PMC	PMC4217412
PMID	25313041
DOI

Davis et al. 2014 (PRJNA245830)