Datasets

Title	A large panel of isogenic APP and PSEN1 mutant human iPSC neurons reveals shared endosomal abnormalities mediated by APP b-CTFs, not Ab [ribosome profiling]
Organism
Number of Samples	12
Release Date	2019/03/15 00:00
Sequencing Types
Protocol Details

Title
Authors	Kwart D,Gregg A,Scheckel C,Murphy EA,Paquet D,Duffield M,Fak J,Olsen O,Darnell RB,Tessier-Lavigne M
Journal	Neuron
Publication Date	2019 Oct 23
Abstract	Familial Alzheimer's disease (fAD) results from mutations in the amyloid precursor protein (APP) and presenilin (PSEN1 and PSEN2) genes. Here we leveraged recent advances in induced pluripotent stem cell (iPSC) and CRISPR/Cas9 genome editing technologies to generate a panel of isogenic knockin human iPSC lines carrying APP and/or PSEN1 mutations. Global transcriptomic and translatomic profiling revealed that fAD mutations have overlapping effects on the expression of AD-related and endocytosis-associated genes. Mutant neurons also increased Rab5+ early endosome size. APP and PSEN1 mutations had discordant effects on Aβ production but similar effects on APP β C-terminal fragments (β-CTFs), which accumulate in all mutant neurons. Importantly, endosomal dysfunction correlated with accumulation of β-CTFs, not Aβ, and could be rescued by pharmacological modulation of β-secretase (BACE). These data display the utility of our mutant iPSCs in studying AD-related phenotypes in a non-overexpression human-based system and support mounting evidence that β-CTF may be critical in AD pathogenesis. Copyright © 2019. Published by Elsevier Inc.
PMC
PMID	31416668
DOI

Kwart et al. 2019 (PRJNA527204)