Datasets

Title	Induction of an alternative 5' leader enhances translation of Inpp5e and resistance to oncolytic virus infection
Organism
Number of Samples	8
Release Date	2019/09/19 00:00
Sequencing Types
Protocol Details

Title
Authors	Hoang HD, Graber TE, Jia JJ, Vaidya N, Gilchrist VH, Xiang X, Li W, Cowan KN, Gkogkas CG, Jaramillo M, Jafarnejad SM, Alain T
Journal	Cell reports
Publication Date	2019 Dec 17
Abstract	Residual cell-intrinsic innate immunity in cancer cells hampers infection with oncolytic viruses. Translational control of mRNA is an important feature of innate immunity, yet the identity of translationally regulated mRNAs functioning in host defense remains ill-defined. We report the translatomes of resistant murine '4T1' breast cancer cells infected with three of the most clinically advanced oncolytic viruses: herpes simplex virus 1, reovirus, and vaccinia virus. Common among all three infections are translationally de-repressed mRNAs, including Inpp5e, encoding an inositol 5-phosphatase that modifies lipid second messenger signaling. We find that viral infection induces the expression of an Inpp5e mRNA variant that lacks repressive upstream open reading frames (uORFs) within its 5' leader and is efficiently translated. Furthermore, we show that INPP5E contributes to antiviral immunity by altering virus attachment. These findings uncover a role for translational control through alternative 5' leader expression and assign an antiviral function to the ciliopathy gene Inpp5e. Copyright © 2019 The Author(s). Published by Elsevier Inc. All rights reserved.
PMC
PMID	31851930
DOI

Hoang et al. 2019 (PRJNA566449)