Brar et al. 2012 (PRJNA149975)

General Details

Title High-resolution view of the yeast meiotic program revealed by ribosome profiling
Organism
Number of Samples 37
Release Date 2012/01/06 00:00
Sequencing Types
Protocol Details

Study Links

Repository Details

SRA SRP009639
ENA SRP009639
GEO
BioProject PRJNA149975

Publication

Title
Authors Brar GA,Yassour M,Friedman N,Regev A,Ingolia NT,Weissman JS
Journal Science (New York, N.Y.)
Publication Date 2012 Feb 3
Abstract Meiosis is a complex developmental process that generates haploid cells from diploid progenitors. We measured messenger RNA (mRNA) abundance and protein production through the yeast meiotic sporulation program and found strong, stage-specific expression for most genes, achieved through control of both mRNA levels and translational efficiency. Monitoring of protein production timing revealed uncharacterized recombination factors and extensive organellar remodeling. Meiotic translation is also shifted toward noncanonical sites, including short open reading frames (ORFs) on unannnotated transcripts and upstream regions of known transcripts (uORFs). Ribosome occupancy at near-cognate uORFs was associated with more efficient ORF translation; by contrast, some AUG uORFs, often exposed by regulated 5' leader extensions, acted competitively. This work reveals pervasive translational control in meiosis and helps to illuminate the molecular basis of the broad restructuring of meiotic cells.
PMC PMC3414261
PMID 22194413
DOI
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)
SRR387871 PRJNA149975 Saccharomyces cerevisiae SK1 Ribo-Seq
SRR387872 PRJNA149975 Saccharomyces cerevisiae SK1 Ribo-Seq
SRR387873 PRJNA149975 Saccharomyces cerevisiae SK1 Ribo-Seq
SRR387874 PRJNA149975 Saccharomyces cerevisiae SK1 Ribo-Seq
SRR387875 PRJNA149975 Saccharomyces cerevisiae SK1 Ribo-Seq
SRR387876 PRJNA149975 Saccharomyces cerevisiae SK1 Ribo-Seq
SRR387877 PRJNA149975 Saccharomyces cerevisiae SK1 Ribo-Seq
SRR387878 PRJNA149975 Saccharomyces cerevisiae SK1 Ribo-Seq
SRR387879 PRJNA149975 Saccharomyces cerevisiae SK1 Ribo-Seq
SRR387880 PRJNA149975 Saccharomyces cerevisiae SK1 Ribo-Seq
SRR387881 PRJNA149975 Saccharomyces cerevisiae SK1 Ribo-Seq
SRR387882 PRJNA149975 Saccharomyces cerevisiae SK1 Ribo-Seq
SRR387883 PRJNA149975 Saccharomyces cerevisiae SK1 Ribo-Seq
SRR387884 PRJNA149975 Saccharomyces cerevisiae SK1 Ribo-Seq
SRR387885 PRJNA149975 Saccharomyces cerevisiae SK1 Ribo-Seq
SRR387886 PRJNA149975 Saccharomyces cerevisiae SK1 Ribo-Seq
SRR387887 PRJNA149975 Saccharomyces cerevisiae SK1 Ribo-Seq
SRR387888 PRJNA149975 Saccharomyces cerevisiae SK1 Ribo-Seq
SRR387889 PRJNA149975 Saccharomyces cerevisiae SK1 Ribo-Seq
SRR387890 PRJNA149975 Saccharomyces cerevisiae SK1 Ribo-Seq
SRR387891 PRJNA149975 Saccharomyces cerevisiae SK1 Ribo-Seq
SRR387892 PRJNA149975 Saccharomyces cerevisiae SK1 Ribo-Seq
SRR387893 PRJNA149975 Saccharomyces cerevisiae SK1 Ribo-Seq
SRR387894 PRJNA149975 Saccharomyces cerevisiae SK1 Ribo-Seq
SRR387895 PRJNA149975 Saccharomyces cerevisiae SK1 Ribo-Seq
SRR387896 PRJNA149975 Saccharomyces cerevisiae SK1 Ribo-Seq
SRR387897 PRJNA149975 Saccharomyces cerevisiae SK1 Ribo-Seq
SRR387898 PRJNA149975 Saccharomyces cerevisiae SK1 Ribo-Seq
SRR387899 PRJNA149975 Saccharomyces cerevisiae SK1 Ribo-Seq
SRR387900 PRJNA149975 Saccharomyces cerevisiae SK1 Ribo-Seq
SRR387901 PRJNA149975 Saccharomyces cerevisiae SK1 Ribo-Seq
SRR387902 PRJNA149975 Saccharomyces cerevisiae SK1 Ribo-Seq
SRR387903 PRJNA149975 Saccharomyces cerevisiae SK1 Ribo-Seq
SRR387904 PRJNA149975 Saccharomyces cerevisiae SK1 Ribo-Seq
SRR387905 PRJNA149975 Saccharomyces cerevisiae SK1 Ribo-Seq
SRR387906 PRJNA149975 Saccharomyces cerevisiae SK1 Ribo-Seq
SRR387907 PRJNA149975 Saccharomyces cerevisiae SK1 Ribo-Seq
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)

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