Xiang et al. 2021 (PRJNA701262)

General Details

Title The molecular basis of coupling between poly(A)-tail length and translational efficiency
Organism
Number of Samples 45
Release Date 2021/02/10 00:00
Sequencing Types
Protocol Details

Study Links

Repository Details

SRA SRP305686
ENA SRP305686
GEO GSE166544
BioProject PRJNA701262

Publication

Title
Authors Xiang K, Bartel DP
Journal eLife
Publication Date 2021 Jul 2
Abstract In animal oocytes and early embryos, mRNA poly(A)-tail length strongly influences translational efficiency (TE), but later in development this coupling between tail length and TE disappears. Here, we elucidate how this coupling is first established and why it disappears. Overexpressing cytoplasmic poly(A)-binding protein (PABPC) in Xenopus oocytes specifically improved translation of short-tailed mRNAs, thereby diminishing coupling between tail length and TE. Thus, strong coupling requires limiting PABPC, implying that in coupled systems longer-tail mRNAs better compete for limiting PABPC. In addition to expressing excess PABPC, post-embryonic mammalian cell lines had two other properties that prevented strong coupling: terminal-uridylation-dependent destabilization of mRNAs lacking bound PABPC, and a regulatory regime wherein PABPC contributes minimally to TE. Thus, these results revealed three fundamental mechanistic requirements for coupling and defined the context-dependent functions for PABPC, which promotes TE but not mRNA stability in coupled systems and mRNA stability but not TE in uncoupled systems. © 2021, Xiang and Bartel.
PMC PMC8253595
PMID 34213414
DOI
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)
SRR13679780 PRJNA701262 Xenopus laevis NA_oocyte RNA-Seq 0.0
SRR13679781 PRJNA701262 Xenopus laevis NA_oocyte RNA-Seq 0.0
SRR13679782 PRJNA701262 Xenopus laevis NA_oocyte RNA-Seq 0.0
SRR13679783 PRJNA701262 Xenopus laevis NA_oocyte RNA-Seq 0.0
SRR13679784 PRJNA701262 Xenopus laevis NA_oocyte RNA-Seq 0.0
SRR13679785 PRJNA701262 Xenopus laevis NA_oocyte RNA-Seq 0.0
SRR13679786 PRJNA701262 Xenopus laevis HeLa RNA-Seq 0.0
SRR13679787 PRJNA701262 Xenopus laevis HeLa RNA-Seq 0.0
SRR13679788 PRJNA701262 Homo sapiens HeLa RNA-Seq 0.0
SRR13679789 PRJNA701262 Homo sapiens HeLa RNA-Seq 0.0
SRR13679779 PRJNA701262 Xenopus laevis HeLa RNA-Seq 0.0
SRR13679790 PRJNA701262 Homo sapiens HeLa RNA-Seq 0.0
SRR13679791 PRJNA701262 Homo sapiens HeLa RNA-Seq 0.0
SRR13679792 PRJNA701262 Homo sapiens HeLa RNA-Seq 0.0
SRR13679793 PRJNA701262 Homo sapiens HeLa RNA-Seq 0.0
SRR13679794 PRJNA701262 Homo sapiens HCT116 RNA-Seq 0.0
SRR13679795 PRJNA701262 Homo sapiens HCT116 RNA-Seq 0.0
SRR13679796 PRJNA701262 Homo sapiens HCT116 Ribo-Seq 0.0
SRR13679797 PRJNA701262 Homo sapiens HCT116 Ribo-Seq 0.0
SRR13679798 PRJNA701262 Xenopus laevis NA_oocyte Ribo-Seq 0.0
SRR13679799 PRJNA701262 Xenopus laevis NA_oocyte Ribo-Seq 0.0
SRR13679800 PRJNA701262 Xenopus laevis NA_oocyte Ribo-Seq 0.0
SRR13679801 PRJNA701262 Xenopus laevis NA_oocyte Ribo-Seq 0.0
SRR13679802 PRJNA701262 Xenopus laevis HeLa Ribo-Seq 0.0
SRR13679803 PRJNA701262 Xenopus laevis HeLa Ribo-Seq 0.0
SRR13679804 PRJNA701262 Xenopus laevis HeLa Ribo-Seq 0.0
SRR13679805 PRJNA701262 Xenopus laevis HeLa Ribo-Seq 0.0
SRR13679806 PRJNA701262 Homo sapiens HeLa Ribo-Seq 0.0
SRR13679807 PRJNA701262 Homo sapiens HeLa Ribo-Seq 0.0
SRR13679808 PRJNA701262 Homo sapiens HeLa Ribo-Seq 0.0
SRR13679809 PRJNA701262 Homo sapiens HeLa Ribo-Seq 0.0
SRR13679810 PRJNA701262 Homo sapiens HeLa Ribo-Seq 0.0
SRR13679811 PRJNA701262 Homo sapiens HeLa Ribo-Seq 0.0
SRR13679812 PRJNA701262 Homo sapiens HCT116 Ribo-Seq 0.0
SRR13679813 PRJNA701262 Homo sapiens HCT116 Ribo-Seq 0.0
SRR13679814 PRJNA701262 Homo sapiens HCT116 Ribo-Seq 0.0
SRR13679815 PRJNA701262 Homo sapiens HCT116 Ribo-Seq 0.0
SRR13679858 PRJNA701262 Homo sapiens HeLa RNA-Seq 0.0
SRR13679859 PRJNA701262 Homo sapiens HeLa RNA-Seq 0.0
SRR13679860 PRJNA701262 Homo sapiens HeLa RNA-Seq 0.0
SRR13679861 PRJNA701262 Homo sapiens HeLa RNA-Seq 0.0
SRR13679862 PRJNA701262 Homo sapiens HeLa RNA-Seq 0.0
SRR13679863 PRJNA701262 Homo sapiens HeLa RNA-Seq 0.0
SRR13679864 PRJNA701262 Homo sapiens HeLa RNA-Seq 0.0
SRR13679865 PRJNA701262 Homo sapiens HeLa RNA-Seq 0.0
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)

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